Isolation and Biological Characterization of Duck Adipose-derived Mesenchymal Stem Cells

doi:10.13560/j.cnki.biotech.bull.1985.2016.08.019

Abstract

Abstract: This study aims to establish in vitro culture system for the poultry adipose-derived stem cells（ADSCs）,to study the biological characteristics and the multi-differentiation potential. Enzyme digestion method was applied to separate Beijing duck 18-day-old embryos ADSCs and to draw the growth curve. ADSCs were identified by RT-PCR,immunofluorescence and flow cytometric analysis. Our results suggested that the ADSCs had solid proliferative activity and were positive for CD29,CD44 and CD105,the rate of CD29+,CD44+,and CD105+ ADSCs was 95.39%,94.53%,98.19%,respectively. ADSCs differentiated into adipocytes and osteoblasts by induced differentiation in vitro. In summary,under appropriate experimental circumstances,Beijing duck embryo ADSCs have a strong self-renewing ability and multi-differentiation potential in vitro,and it can be an ideal type of seed cell for cellular transplantation therapy.

Key words: adipose-derived stem cells, isolation, in vitro directional differentiation, multi-differentiation potential, the biological characteristics

LIU Xue-ting, YUAN Hong-yi, ZHANG Ming-hai, GUAN Wei-jun. Isolation and Biological Characterization of Duck Adipose-derived Mesenchymal Stem Cells[J]. Biotechnology Bulletin, 2016, 32(8): 122-128.

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