Technology of Artificial microRNA and Its Application in Breeding Disease-resistant Plants

doi:10.13560/j.cnki.biotech.bull.1985.2017.02.007

Abstract

Abstract: Endogenous microRNAs（miRNAs）are recognized as important regulators of gene expression in eukaryotes. Artificial microRNA（amiRNA）is an effective strategy of silencing endogenous gene based on natural miRNAs. Using amiRNA to replace the miRNA/miRNA* sequence in plant’s endogenous miRNA precursor,the generated amiRNA match the target gene,specifically suppressing the expression of target gene. Here,we summarize the synthetic process and action mechanism of natural miRNA,the basic design principles and construction methods of amiRNA,and the application of artificial miRNA technique in breeding disease-resistant plant.

Key words: miRNA, artificial miRNA, transgenic, disease-resistant breeding

LIN Yi-hua, ZHENG Tao, GAO San-ji, CHEN Zhen-dong. Technology of Artificial microRNA and Its Application in Breeding Disease-resistant Plants[J]. Biotechnology Bulletin, 2017, 33(2): 47-52.

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