Screening and Identification of Two Aerobic Denitrifying Phosphorus-accumulating Strains,and Denitrifying Biological Phosphorus Removal

doi:10.13560/j.cnki.biotech.bull.1985.2017.03.017

Abstract

Abstract: It is aimed to screen highly-efficient aerobic denitrification bacterium simultaneously removing nitrogen and phosphorus（P）from the piggery wastewater sample in a pig farm of Fuzhou. Two effective strains,named as N1 and N2,were acquired by culturing with medium for denitrification strain,then isolating with the BTB streak plate,further screening with the blue- and white-colored spot,and finally dyeing with metachromatic granules and PHB（poly-β-hydroxybutyrate）granule. By 16S rRNA gene sequence analyses,two strains were identified as Achromobacter sp. and Brevundimonas sp.,respectively. Combined strain FIM-1 was formulated with N1 and N2. The effectiveness of their removing the nitrogen and phosphorus in artificial wastewater and eutrophic water was investigated. The results showed that the removal rate of nitrogen by N1,N2,and FIM-1 at 40 h was 9.20%,17.16%,and 32.84% respectively,and the removal rate of total P at 18 h was 14.04%,8.77%,and 28.95%,respectively,indicating that FIM-1 performed better than the single ones.

Key words: aerobic denitrification, phosphorus-accumulating organisms（PAOs）, nitrogen and phosphorus removal, eutrophication

NIE Yi-lei, JIA Wei, ZENG Yan-bing, LUO Li-jing, CHEN Xing-wei, ZHUANG Hong, CHEN Hong. Screening and Identification of Two Aerobic Denitrifying Phosphorus-accumulating Strains,and Denitrifying Biological Phosphorus Removal[J]. Biotechnology Bulletin, 2017, 33(3): 116-121.

References

[1] 秦伯强, 高光, 朱广伟, 等. 湖泊富营养化及其生态系统响应[J]. 科学通报, 2013, 58（10）：855-864.
[2] 赵永宏, 邓祥征, 战金艳, 等. 我国湖泊富营养化防治与控制策略研究进展[J]. 环境科学与技术, 2010, 33（3）：92-98.
[3] 徐亚同. 水中氮磷的处理[M]. 上海：华东师范大学出版社, 1996：130-133.
[4] Robertson LA, Kuenen JG. Aerobic denitrification：a controversy revived[J]. Archives of Microbiology, 1984, 139（4）：351-354.
[5] Robertson LA, Van Niel EWJ, Torremans RAM, et al. Simultaneous nitrification and denitrification in aerobic chemostat cultures of Thiosphaera pantotropha[J]. Applied and Environmental Microbiology, 1988, 54（11）：2812-2818.
[6] Takaya N, Catalan-Sakairi MAB, Sakaguchi Y, et al. Aerobic denitrifying bacteria that produce low levels of nitrous oxide[J]. Appl Environ Microbio, 2003, 69（6）：3152-3157.
[7] 杨金水. 资源与环境微生物学实验教程[M]. 北京：科学出版社, 2014：36-46.
[8] 东秀珠, 蔡妙英编著. 常见细菌系统鉴定手册[M]. 北京：科学出版社, 2001.
[9] Serafim LS, Lemos PC, Levantesi C, et a1. Methods for detection and visualization of intracellular polymers stored by polyphosphate-accumulating microorganisms[J]. Journal of Microbiological Methods, 2002, 5 l（1）：14-18.
[10] 朱旭芬. 现代微生物学实验技术[M]. 杭州：浙江大学出版社, 2011：141-142.
[11] 国家环境保护总局《水和废水监测分析方法》编委会. 水和废水监测分析方法[M]. 北京：中国环境科学出版社, 2002：244-248.
[12] 王薇, 蔡祖聪, 钟文辉, 等. 好氧反硝化菌的研究进展[J]. 应用生态学报, 2007, 18（11）：2618-2625.
[13] Su JJ, Liu BY, Liu CY. Comparison of aerobic denitrification under high oxygen atmosphere by Thiosphaera pantotropha ATCC 35512 and Pseudomonas stutzeri SU2 newly isolated from the activated sludge of a piggery wastewater treatment system[J]. Journal of Applied Microbiology, 2001, 90（3）：457-462.
[14] Joo HS, Hirai M, Shoda M. Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis No. 4[J]. Journal of Bioscience and Bioengineering, 2005, 100（2）：184-191.
[15] Patureau D, Zumstein E, Delgenes JP, et al. Aerobic denitrifiers isolated from diverse natural and managed ecosystems[J]. Microbial Ecology, 2000, 39（2）：145-152.
[16] 杨希, 刘德立, 邓灵福, 等. 蜡状芽孢杆菌好氧反硝化特性研究[J]. 环境科学研究, 2008, 21（3）：155-159.
[17] 于大禹, 郭威, 张金榜, 等. 好氧反硝化菌的筛选及其脱氮除磷性质的研究[J]. 微生物学通报, 2009, （4）：598-603.
[18] Ma T, Chen Q, Gui M, et al. Simultaneous denitrification and phosphorus removal by Agrobacterium sp. LAD9 under varying oxygen concentration[J]. Applied Microbiology and Biotechnology, 2016, 100（7）：1-10.
[19] 马放, 杨菲菲, 张倩, 等. 一株高效反硝化聚磷菌的筛选及脱氮除磷效能[J]. 哈尔滨工业大学学报, 2011, 43（12）：42-47.
[20] 安健, 伏光辉, 阮记明, 等. 反硝化除磷菌筛选及其特性研究[J]. 微生物学通报, 2012, 39（2）：162-167.
[21] 赵林林, 王海燕, 杨慧芬, 等. 废水反硝化除磷技术研究进展[J]. 化工环保, 2011, 31（1）：38-41.
[22] 李勇智, 彭永臻, 王淑滢, 等. 强化生物除磷体系中的反硝化除磷[J]. 中国环境科学, 2003, 23（5）：543-546.
[23] 李军, 彭永臻, 杨秀山, 等. 序批式生物膜法反硝化除磷特性及其机理[J]. 中国环境科学, 2004, 24（2）：219-223.
[24] 方茜, 张朝升, 张可方, 等. 溶解氧对反硝化聚磷菌的影响研究[J]. 中国给水排水, 2008, 24（1）：35-39.
[25] 潘玉瑾, 刘芳, 孟爽, 等. 好氧反硝化菌P. chengduensis ZPQ2的筛选及其反硝化条件优化[J]. 环境工程, 2016, 34（1）：41-46.
[26] 方茜, 张朝升, 张立秋, 等. 曝气量对同时硝化/反硝化除磷工艺效能的影响[J]. 中国给水排水, 2014, 30（21）：14-18.
[27] 吕冬梅, 彭永臻, 赵伟华, 等. A 2 O-BAF工艺反硝化聚磷效果的影响因素[J]. 中国环境科学, 2015, 35（11）：3266-3274.
[28] 孙庆花, 于德爽, 张培玉, 等. 1株海洋异养硝化-好氧反硝化菌的分离鉴定及其脱氮特性[J]. 环境科学, 2016, 37（2）：647-654.
[29] Yang M, Xue D, Meng F. The effects of salinity on coupled nitrification and aerobic denitrification in an estuarine system[J]. Water Sci Technol, 2015, 71（11）：1718-1726.
[30] Bang DY, Watanabe Y, An experimental study on aerobic denitrification with polyvinyl alcohol as a carbon source in biofilms[J]. Water Science & Technology, 2015, 32（8）：235-242.