Screening and Denitrification Characteristics of an Aerobic Denitrifying-Heterotrophic Nitrification Bacterium

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.021

Abstract

Abstract: Aiming at the fact that the degradation of NO₃-N and NO₂-N in leachate is difficult, an aerobic denitrifying bacterium HN, preliminary identified as Pseudomonas, was isolated from an aerobic biological reactor landfill. Based on its degradation capacities to different nitrogen sources, we studied the denitrification characteristics of HN and optimized the conditions for denitrification. The results showed that under aerobic conditions and potassium nitrate as sole nitrogen source, the removal efficiency of nitrogen in nitrate was up to 95.44% at 96 h；when using ammonium sulfate as sole nitrogen source, HN could remove 85.14% nitrogen in ammonia at 60 h；when the carbon source was ethanol, C/N was 7:1, initial pH was 7.5, the temperature was 35℃ and inoculation amount was 10%, HN had the highest removal efficiency.

Key words: biological denitrification, aerobic denitrification, heterotrophic nitrification, Pseudomonas

Lian Hongmin, Qiu Zhongping, He Kunming, Zhou Wenxiu. Screening and Denitrification Characteristics of an Aerobic Denitrifying-Heterotrophic Nitrification Bacterium[J]. Biotechnology Bulletin, 2015, 31(6): 138-143.

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