Abstract: This work is to establish a regenerative system of Sopatholobus suberechtus tissue and determine the dynamic changes of endogenous hormones during the regeneration process. The enzyme-linked immunosorbent assay（ELISA）was used to determine and analyze the dynamic changes of four endogenous hormones（IAA,ABA,ZT,and GA₃）contents during the callus induction and differentiation. As results,the content of IAA always kept at a high level and reached maximum when the callus growth was the most exuberant in the process of callus induction;while the contents of ABA,ZT and GA₃ steadily remained at low levels;and IAA/ABA presented an important regulatory role on callus induction and growth. Further,the content of IAA decreased at first and rose then,reached the highest with 1.15-1.87 μg/g while differentiated to bud,and higher than that in callus induction period. ZT and ABA showed rising trend at first and then down later,while GA₃ tended to rise continuously. Also IAA/ABA decreased at first and rose then,playing an important role in promoting bud differentiation and growth. GA₃/ABA and ZT/ABA decreased at first and rose then,and promoted the successful differentiation of callus tissue to the adventitious bud in synergistic effects. In conclusion,the four kinds of endogenous hormones have important influences on callus induction and adventitious bud differentiation,the appropriate concentration and ratio of them may effectively promote the explants dedifferentiation and re-differentiation,and they synergistically promote cell division,differentiation and growth.

Key words: Sopatholobus suberechtus Dunn, callus induction, differentiation culture, plant endogenous hormones