Analysis of Transcriptional Activity and Interaction Proteins of WRKY31 Transcription Factor in Arabidopsis thaliana

doi:10.13560/j.cnki.biotech.bull.1985.2017-0918

Abstract

Abstract: In order to deepen our understanding on functionally-unknown members in AtWRKY gene family,and to explore the roles and regulatory mechanisms of WRKY genes in Arabidopsis thaliana in response to abiotic stresses,we chose to study AtWRKY31 gene. First,we performed the subcellular localization assay of AtWRKY31 and analyzed its transcriptional activity. Then,we examined the expression changes of AtWRKY31 under different stress treatments for 1 h,3 h and 12 h through qRT-PCR. At last,we used yeast two-hybrid（Y2H）and bimolecular fluorescence complementation（BiFC）assays to screen and validate the interacting partners of WRKY31. The results showed that AtWRKY31 protein was located in the nucleus and acted as a transcriptional repressor. qRT-PCR showed that the expression of WRKY31 was significantly inhibited under heat,low potassium and low nitrogen treatments. Through yeast two-hybrid and BiFC assays,WRKY31 protein was identified to interact with itself,WRKY6 and WRKY42,respectively. Conclusively,WRKY31 in Arabidopsis thaliana,as a transcriptional repressor,may be involved in the regulation of some abiotic stress responses by the interactions with transcriptional factor WRKY6 and WRKY42.

Key words: Arabidopsis thaliana, WRKY31, subcellular localization, yeast two-hybrid, bimolecular fluorescence complementation

WANG Yi-qiao, ZHAO Xin-jie, NIU Fang-fang, GUO Xiao-hua, YANG Bo, JIANG Yuan-qing. Analysis of Transcriptional Activity and Interaction Proteins of WRKY31 Transcription Factor in Arabidopsis thaliana[J]. Biotechnology Bulletin, 2018, 34(5): 101-109.

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