Probe into Application of CRISPR/Cas9 Technology in Epigenome

doi:10.13560/j.cnki.biotech.bull.1985.2018-0358

Abstract

Abstract: CRISPR/Cas9（clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins）technology is the most widely used novel gene editing tool in the past five years. With the advantages of low cost,high efficiency,multi-site target and predictable off-target effects,it is becoming a powerful tool for designing genomes in different organisms. The continuous development of epigenetics challenges the central principles in molecular biology,including the modification of bases and histones in DNA and the crucial role of non-coding RNA in the regulation of genome and chromatin structure. The alteration of CRISPR/Cas9 system may modify the epigenome. This article summarizes the application of CRISPR/Cas9 system in modifying epigenome,discusses the problems in the directional modification of the genome,and provides references for the application of CRISPR/Cas9 system in the field of genome editing.

Key words: CRISPR/Cas9, gene editing, epigenome, epigenetic regulation

HE Shuai, WANG Shu-hui, SUN Xiu-zhu. Probe into Application of CRISPR/Cas9 Technology in Epigenome[J]. Biotechnology Bulletin, 2018, 34(5): 17-21.

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