Biotechnology Bulletin ›› 2019, Vol. 35 ›› Issue (5): 35-41.doi: 10.13560/j.cnki.biotech.bull.1985.2018-1102

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Functional Analysis of FAD7 Gene in Sweet Sorghum

SONG Yu-shuang, SUI Na   

  1. Shandong Normal University,School of Life Sciences,Jinan 250014
  • Received:2018-12-27 Online:2019-05-26 Published:2019-05-23

Abstract: Here firstly we isolated and cloned ω-3 fatty acid desaturase(FAD7)gene from sweet sorghum inbred line M81-E. Then we constructed an expression vector to infect Arabidopsis thaliana and obtained the homozygous plants with SbFAD7overexpression. It was preliminarily indicated that the gene played an important role in increasing the cold resistance of plant by measuring and analyzing the contents of Fo,Fv/Fm,electrical conductivity,MDA,superoxide anion radical and hydrogen peroxide content in wild and overexpressed plants under low temperature treatment.

Key words: sweet sorghum, cold resistance, ω-3 fatty acid desaturase;