Comparison of Mitochondrial Genome Between A1 Cytoplasmic Male Sterile Line and Maintainer Line of Sorghum bicolor

doi:10.13560/j.cnki.biotech.bull.1985.2019-0089

Abstract

Abstract: Mitochondrial genomic translocation is an important genetic mechanism for cytoplasmic male sterility（CMS）in crops. The main purpose of this study is to explore the translocation region of mitochondrial genomes by comparing the mitochondrial genome between A1 cytoplasmic male sterile line and maintainer line of Sorghum bicolor,and to lay a foundation for cloning related genes of sorghum A1 cytoplasmic male sterility. A1 cytoplasmic male sterile line Tx623A and its maintainer line Tx623B of sorghum were used in this experiment. Their mitochondrial genome sequencing data from Illumina Hiseq（next generation sequencing）and PacBio seq（third generation sequencing）were assembled,and then,the differences of genome structures and genes between the two lines were compared and analyzed. The results showed that the mitochondrial genome sizes of Tx623A and Tx623B were 449 727 bp and 452 772 bp,respectively. The predicted open reading frames（ORFs）were 147 and 145,among which the number of the specific genes for Tx623A were 8 and 6 for Tx623B. Importantly,a 57 kb genome structural variation（SV）region was found after comparing the two mitochondrial genomes,and which may be related to A1 cytoplasmic male sterility. Collectively,our results provide genomic information for cloning related genes of sorghum A1 cytoplasmic male sterility.

Key words: Sorghum bicolor, sterile line, maintainer line, mitochondrial genome, cytoplasmic male sterility

WANG Ping, CONG Ling, WANG Chun-yu, ZHU Zhen-xing, A Ashok KUMAR, ZHANG Li-xia, LU Xiao-chun. Comparison of Mitochondrial Genome Between A1 Cytoplasmic Male Sterile Line and Maintainer Line of Sorghum bicolor[J]. Biotechnology Bulletin, 2019, 35(5): 42-47.

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