Probe on the Catalytic Mechanism of Glycosyltransferase in Grapefruit for Quercetin and Its Analogues

doi:10.13560/j.cnki.biotech.bull.1985.2019-0057

Abstract

Abstract: Using Glycosyltransferase（FGT）in grapefruit as catalytic enzyme,UDP-glucose,UDP-N-acetyl-D-glucosamine,and UDP-mannose as glycosyl donor,quercetin and naringin as glycosylation receptor,glycosylation was studied. The reaction of the product was detected by high performance liquid chromatography,mass spectrometry and nuclear magnetic resonance（NMR）hydrogen spectrum. The glycosylation reaction of quercetin with UDP-glucose and UDP-N-acetyl-D-glucosamine was confirmed. The molecular weight of quercetin glycoside was 464 and 505,respectively. According to the results of nuclear magnetic resonance,the structures of the products were quercetin-3-O-beta-D glucoside and quercetin-3-O-beta-D-N-acetyl-D-glucosamine. It was verified that FGT enzyme was used to modify quercetin glycosylation.

Key words: glycosylation, quercetin, quercetin glycoside

YE Jing-si, DOU Wen-fang, WANG Cheng. Probe on the Catalytic Mechanism of Glycosyltransferase in Grapefruit for Quercetin and Its Analogues[J]. Biotechnology Bulletin, 2019, 35(7): 121-128.

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