Effects of PMT1 and PMT3 Double-gene Deficiency on the Lifespan of Saccharomyces cerevisiae

doi:10.13560/j.cnki.biotech.bull.1985.2017-0847

Abstract

Abstract: We herein aimed to explore the effects of the PMT3 of protein O-mannosyltransferase（PMT）single-gene deficiency,and PMT1 and PMT3 double-gene deficiency on the replicative lifespan of Saccharomyces cerevisiae. PMT3 deficiency strain（pmt3Δ）was constructed through polymerase chain reaction（PCR）-mediated one-step gene disruption,and PMT1 and PMT3 double-gene deficiency strain（pmt1Δpmt3Δ）was prepared based on homologous recombination. The replicative lifespan of yeast cells were counted by separating daughter cells from mother cells using a manual micromanipulator equipped with a fiber-optic needle under the microscope. The replicative lifespan of the pmt3Δ yeast strain（24 generations）and pmt1Δpmt3Δ strain（25 generations,P > 0.05）exhibited no significant difference,compared with that of wild-type yeast strain（26 generations）（P > 0.05）,i.e.,there was no statistical significance among the differences. Conclusively,PMT3 single-gene deficiency and PMT1 and PMT3 double-gene deficiency exert no evident effects on the lifespan of yeast cells.

Key words: Saccharomyces cerevisiae, cell lifespan, budding, glycosylation

CUI Hong-jing, ZHOU Tao, QIU Kun-pei, SONG Hao-chang, LIU Xin-guang. Effects of PMT1 and PMT3 Double-gene Deficiency on the Lifespan of Saccharomyces cerevisiae[J]. Biotechnology Bulletin, 2018, 34(4): 139-143.

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