Study on the Degradation of PAHs by Bacillus thuringiensis

doi:10.13560/j.cnki.biotech.bull.1985.2018-0879

Abstract

Abstract: In order to study the performance of Bacillus thuringiensis degrading anthracene,pyrene and fluorene at different temperatures,pH and qualities of PAHs,a single factor experiment and a response surface analysis were combined,Soxhlet extraction method was applied to determine the degradation absorbance value using PAHs as the sole carbon source and activated carbon as an adsorption carrier. The experimental results were analyzed via Box-Behnken design in software Design Expert 8.0.6.1. The results showed that the interaction between temperature and pH presented significant effects on the degradation of anthracene and pyrene,and the interaction between temperature and PAHs quality significantly affected the degradation of fluorene. In the optimized experiments,the predicted degradation rates of anthracene,pyrene,and fluorene under respective optimized conditions were 92.13%,87.08%,and 83.56% respectively,the relative error between the predicted value and the actual value 91.60%,86.62%,and 82.95% were ±0.58%,±0.53%,and ±0.73%.The relative error of the three optimization models were all within 2%,indicating that the three experimental models could be used to analyze and predict the degradation effect of B. thuringiensis for anthracene,pyrene,and fluorene.

Key words: Bacillus thuringiensis, degradation performance, response surface analysis, anthracene, fluorene, pyrene

LUO Xiao-fang, CHEN Li-hua, XIA Miao-miao, XU Shu-juan, XIONG Mei. Study on the Degradation of PAHs by Bacillus thuringiensis[J]. Biotechnology Bulletin, 2019, 35(9): 125-133.

References

[1] 李嘉康, 宋雪英, 崔小维, 等. 土壤中多环芳烃源解析技术研究进展[J]. 生态科学, 2017, 36(5):223-231.
[2] 杨发忠, 颜阳, 张泽志, 等. 多环芳烃研究进展[J]. 云南化工, 2005, 32(2):44-48.
[3] 蹇兴超. 多环芳烃(PAH)的污染[J]. 环境保护, 1995(10):31-33.
[4] 温志良, 莫大伦. 土壤污染研究现状与趋势[J]. 土壤污染与防治, 2000, 22(3):56-58.
[5] 程晓暄, 刘昱, 张枝焕, 等. 土壤中多环芳烃微生物降解能力模拟[J]. 环境科学研究, 2017, 30(9):1373-1381.
[6] 包贞, 潘志彦, 杨晔, 等. 环境中多环芳烃的分布及降解[J]. 浙江工业大学学报, 2003(5):58-63, 74.
[7] 王涛, 蓝慧, 田云, 等. 多环芳烃的微生物降解机制研究进展[J]. 化学与生物工程, 2016(2):8-14.
[8] 丁克强, 骆永明. 多环芳烃污染土壤的生物修复[J]. 土壤, 2001, 33(4):169-178.
[9] 李春玉. 多环芳烃的土壤降解特性及其影响因子研究[D]. 南京:南京农业大学, 2008.
[10] 孙海波. 多环芳烃降解菌的筛选、鉴定、降解特性及邻苯二酚-2, 3-双加氧酶的初步研[D]. 济南:山东大学, 2009.
[11] 王艳, 辛嘉英, 宋昊, 等. 生物降解萘的研究进展[J]. 中国生物工程杂志, 2009, 29(9):119-124.
[12] 温洪宇, 廖银章, 李旭东. 微生物降解多环芳烃的研究进展[J]. 微生物学杂志, 2005, 25(6):73-75.
[13] 肖嘉俊, 陆贻通. 土壤微生物降解多环芳烃菲的研究进展[J]. 科技通报, 2009, 25(5):673-679.
[14] 史兵方, 杨秀培, 张有会, 等. 土壤中多环芳烃污染的研究进展[J]. 安徽农业科学, 2007, 35(6):1735-1737.
[15] 金卫根, 陈传红, 周亚平, 等. 两株多环芳烃降解菌的筛选与生化鉴定[J]. 农业科技与信息, 2015, 17:86-89.
[16] 童乐, 何丽媛, 郭楚玲, 等. 以农作物秸秆固定化混合菌去除原油[J]. 环境工程学报, 2016, 10(5):2707-2713.
[17] 王冬梅, 陈丽华, 雒晓芳, 等. 鼠李糖脂与菌剂对原油污染土壤的联合修复[J]. 环境工程学报, 2014, 8(11):5003-5009.
[18] 王欣, 王金翠, 殷晓梅, 等. 乙酰甲胺磷UV—TiO2/类Fenton光催化降解过程的响应面法优化[J]. 应用化工, 2013, 42(1):33-40.
[19] 刁硕, 王红旗, 吴枭雄, 等. 基于响应面法优化一株低温耐盐芘降解菌共代谢条件的研究[J]. 中国环境科学, 2017, 37(1):345-351.
[20] 朱天辉, 杨佐忠. 枯草芽孢杆菌菌种退化及其控制[J]. 西南林业大学学报:自然科学版, 2000, 20(1):31-35.
[21] 秦智, 于松翠, 刘波, 等.固定化枯草芽孢杆菌的制备方法及采用固定化枯草芽孢杆菌处理污水的方法:CN, CN103160492A[P]. 2013.
[22] 蒋丽娟. 降解多环芳烃高效菌及生物活性炭的研制[D]. 武汉:武汉科技大学, 2011.
[23] 杨婧. 1株苏云金芽孢杆菌的生物活性研究[D]. 沈阳:辽宁师范大学, 2011.
[24] 黄兴如, 张彩文, 张瑞杰, 等. 多环芳烃降解菌的筛选、鉴定及降解特性[J]. 微生物学通报, 2016, 43(5):965-973.
[25] 朱玮, 赵兵, 王晓东, 等. 生物农药苏云金芽孢杆菌的研究进展[J]. 过程工程学报, 2004, 3:282-288.
[26] 李莉, 张赛, 何强, 等. 响应面法在试验设计与优化中的应用[J]. 实验室研究与探索, 2015, 34(8):41-45.
[27] 陈晓鹏, 易筱筠, 陶雪琴, 等. 石油污染土壤中芘高效降解菌群的筛选及降解特性研究[J]. 环境工程学报, 2008, 2(3):413-417.
[28] Lu J, Guo C, Zhang M, et al.Biodegradation of single pyrene and mixtures of pyrene by a fusant bacterial strain F14[J]. Int Biodeter Biodegr, 2014, 87:75-80.
[29] Liu W, Yu YY, Yang RZ, et al.Optimization of total flavonoid compound extraction from gynura medica leaf using response surface methodology and chemical composition analysis[J]. Int J Mol Sci, 2010, 11(11):4750-4763.
[30] Ashan SE, Miganad T, Ariff A, et al.Optimization of enzymatic synthesis of palm-based kojic acid ester using response surface methodology[J]. J Oleo Sci, 2009, 58(10):503-510.
[31] Xiao WH, Han LJ, Shi B.Optimization of microwaveassisted extraction of flavonoid from Radix Astragali using response surface methodology[J]. Sep Sci Technol, 2008, 43(3):671-681.
[32] Nadarajah N, Singh A.De-emulsification of petroleum oil emulsion by a mixed bacterial culture[J]. Process Biochemistry, 2002, 37:1135-1141.
[33] Sarkar D, Fergusonm, Datta R, et al.Bioremediation of petroleum hydrocarbons in contaminated soils:comparison of biosolids addition, carbon supplementation, and monitored natural attenuation[J]. Environmental Pollution, 2005, 136:187-195.