Analysis of Salt Tolerance of Transgenic BpLTP4 Tobacco

doi:10.13560/j.cnki.biotech.bull.1985.2020-0498

Abstract

Abstract:

The BpLTP4 gene with CDS of 357 bp was cloned from Betula platyphylla Suk. The BpLTP4 protein was a hydrophobic and had a potential transmembrane region. The tertiary structure of the protein contained a hydrophobic cavity. The protein had 8 Cys residue structures unique specifically to LTP family genes. BpLTP4 was closely related to LTP salt- tolerant genes of other species. qRT-PCR analysis showed that NaCl stress treatment for 48 h highly induced BpLTP4 expression. BpLTP4 overexpressed tobacco transgenic lines were obtained by leaf disc method. After 150 mmol/L NaCl stress treatment of transgenic and wild-type（WT）lines,it was found that the transgenic lines had higher salt tolerance than the WT,and the average germination rate was 5.6 times that of WT. The root length was 1.099 times that of WT. DAB,NBT,Evans blue staining were lighter than WT,POD activity was 0.28 times higher than WT,and SOD activity was 0.12 times higher than WT. In response to salt stress treatment,BpLTP4 improves the salt tolerance of transgenic grass by increasing the protective enzyme activity,removing reactive oxygen species,and maintaining cell integrity.

Key words: Betula platyphylla Suk, BpLTP4, salt tolerance analysis, tobacco

SHI Jing-jing, GUO Yi-ping, YU Ying, ZHOU Mei-qi, WANG Chao. Analysis of Salt Tolerance of Transgenic BpLTP4 Tobacco[J]. Biotechnology Bulletin, 2020, 36(12): 34-41.

Figures/Tables 10

References 26

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