Screening and Performance Analysis of High-temperature Phosphate-solubilizing Microorgams in High Temperature Compost with Phosphate Rock and Discarded Vinasse Lost Grains

doi:10.13560/j.cnki.biotech.bull.1985.2019-1150

Abstract

Abstract: In order to solve the problem of the phosphorus dissolution in the high temperature environment,the phosphate-solubilizing microorgams were screened from high temperature compost. Using inorganic phosphorus selective medium,5 strains of high temperature resistant and phosphate-solubilizing microorganisms（bacteria GDB1-2;fungus GDF1-3）were isolated and screened from the high-temperature compost samples added with phosphate rock and lost grains. The 5 strains were identified by morphological and molecular biology and solubilized-phosphorus capability. The results showed that the 5 isolates were Bacillus subtilis,Bacillus licheniformis,Lichtheimia ramosa,Aspergillus fumigatus and Aspergillus nidulans. The phosphorus solubilization curve of the 5 strains showed that the highest phosphorus content of each strain ranged from 136.85 to 174.33 μg/mL. It was found that each strain had a wide range of high temperature resistance and grew between 40-60℃,but its ability to dissolve phosphorus reached a maximum at 50℃. Under the initial pH of 5-9,all the 5 strains maintained a certain ability of dissolving phosphorus. The research results provide materials for the subsequent development of high-temperature environmental microbial resources,and have a good application and promotion prospects.

Key words: high-temperature phosphate-solubilizing microorganism, high-temperature compost, screening, solubilized-phosphorus capability

ZHANG Rui-rui, QIU Shu-yi, ZHOU Shao-qi, WANG Xue-li. Screening and Performance Analysis of High-temperature Phosphate-solubilizing Microorgams in High Temperature Compost with Phosphate Rock and Discarded Vinasse Lost Grains[J]. Biotechnology Bulletin, 2020, 36(5): 110-119.

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