Identification and Degradation Characteristics of Microbial Community Degrading Phenanthrene from Surface Sediments

doi:10.13560/j.cnki.biotech.bull.1985.2019-1044

Abstract

Abstract: A novel aerobic microbial consortium with microbial degradation was enriched and isolated from river surface sediments,and its microbial community structure was identified and its degradation characteristics were studied. The microbial community structure of the consortium was analyzed by 16S rRNA gene high throughput sequencing,and the optimal growth and degradation conditions were determined by single factor experiment. The results showed that the consortium mainly included 83.35% of Diaphobiobacter,7.46% of Pseudomonas,1.67% of Castellaellasti,1.65% of Aquamicrobium,etc. The consortium degraded > 95.78% of 100 mg/L phenanthrene in 60 h,remained its efficient degradation rate between pH5 and pH11,and optimal growth temperature was 35℃.The effect on the consortium was small,and the degradation rate was still maintained at 97.26% when the salinity was 1%. The phenanthrene with a concentration of 400 mg/L was degraded in 7 d with the degradation rate 96.36%.The consortium not only has the ability of high-efficiency degradation,but also grows in a wide range of conditions and has strong compressive ability,which may provide new germplasm resources for the bioremediation of polycyclic aromatic hydrocarbons（PAHs）.

Key words: phenanthrene, microbial community structure, 16S rRNA gene high throughput sequencing, degradation kinetics

ZHANG Yong-min, WANG Tian-hui, WANG Ping. Identification and Degradation Characteristics of Microbial Community Degrading Phenanthrene from Surface Sediments[J]. Biotechnology Bulletin, 2020, 36(6): 128-135.

References

[1] 宋世杰, 黄韬, 周胜, 等. 博斯腾湖流域沉积物中多环芳烃的时空分布、来源及生态风险评价[J]. 环境科学学报, 2019, 39(8):2780-2790.
[2] Hadibarata T, Yusoff AR, Aris A, et al.Identification of naphthalene metabolism by white rot fungus Armillaria sp. F022[J]. J Environ Sci, 2012, 24(4):728-732.
[3] Sukhdhane KS, Pandey PK, Ajima MNO, et al.Isolation and characterization of phenanthrene-degrading bacteria from PAHs contaminated mangrove sediment of thane creek in Mumbai, India[J]. Polycyclic Aromatic Compounds, 2019, 39(1):73-83.
[4] Kuppusamy S, Thavamani P, Megharaj M, et al.Polyaromatic aromatic hydrocarbon(PAH)degradation potential of a new acid tolerant, diazotrophic P-solubilizing and heavy metal resistant bacterium Cupriavidus sp. MTS-7 isolated from long-term mixed contaminated soil[J]. Chemosphere, 2016, 162(11):31-39.
[5] Sun S, Wang Y, Zang T, et al.A biosurfactant-producing Pseudomonas aeruginos S5 isolated from coking wastewater and its application for bioremediation of polycyclic aromatic hydrocarbons[J]. Bioresource Technology, 2019, 281(6):421-428.
[6] Kamyabi A, Nouri H, Moghimi H.Characterization of pyrene degradation and metabolite identification by Basidioascus persicus, and mineralization enhancement with bacterial-yeast co-culture[J]. Ecotoxicol Environ Saf, 2018, 163(11):471-477.
[7] Li XZ, Pan YS, Hu S, et al.Diversity of phenanthrene and benz[a]anthracene metabolic pathways in white rot fungus Pycnoporus sanguineus 14[J]. International Biodeterioration & Biodegradation, 2018, 134(10):25-30.
[8] Bacosa HP, Inoue C.Polycyclic aromatic hydrocarbons(PAHs)biodegradation potential and diversity of microbial consortia enriched from tsunami sediments in Miyagi, Japan[J]. Journal of Hazardous Materials, 2015, 283(2):689-697.
[9] Bharatkumar PA, Mahala K, Jain K, et al.Development of mixed bacterial cultures DAK11 capable for degrading mixture of polycyclic aromatic hydrocarbons(PAHs)[J]. Bioresource Technology, 2018, 253(4):288-296.
[10] Wu M, Chen L, Tian Y, et al.Degradation of polycyclic aromatic hydrocarbons by microbial consortia enriched from three soils using two different culture media[J]. Environmental Pollution, 2013, 178(7):152-158.
[11] 胡文. 马鞍山河流沉积物多环芳烃分布特征及其吸附机理研究[D]. 马鞍山:安徽工业大学, 2018.
[12] 钟磊. 菲高效降解菌的分离、鉴定及其在菲污染土壤修复中的应用研究[D]. 南京:南京农业大学, 2009.
[13] Oyehan TA, Al-Thukair AA.Isolation and characterization of PAH-degrading bacteria from the eastern province, Saudi Arabia[J]. Marine Pollution Bulletin, 2017, 115(1-2):39-46.
[14] Klankeo P, Nopcharoenkul W, Pinyakong O.Two novel pyrene-degrading Diaphorobacter sp. and Pseudoxanthomonas sp. isolated from soil[J]. Journal of Bioscience & Bioengineering, 2009, 108(6):488-495.
[15] 段佩玲, 王振宇, 党玉姣, 等. 一株Diaphorobacter属细菌对苯酚的降解特性和代谢途径[J]. 环境科学与技术, 2010, 33(8):33-36.
[16] Yang C, Li R, Song Y, et al.Identification of the biochemical degradation pathway of triazophos and its intermediate in Diapho-robacter sp. TPD-1[J]. Current Microbiology, 2011, 62(4):1294-1301.
[17] Kuppusamy S, Thavamani P, Megharaj M, et al.Isolation and characterization of polycyclic aromatic hydrocarbons(PAHs)degrading, pH tolerant, N-fixing and P-solubilizing novel bacteria from manufactured gas plant(MGP)site soils[J]. Environmental Technology & Innovation, 2016, 6(11):204-219.
[18] Ma Z, Liu J, Dick RP, et al.Rhamnolipid influences biosorption and biodegradation of phenanthrene by phenanthrene-degrading strain Pseudomonas sp. Ph6[J]. Environmental Pollution, 2018, 240(9):359-367.
[19] Chebbi A, Hentati D, Zaghden H, et al.Polycyclic aromatic hydrocarbon degradation and biosurfactant production by a newly isolated, Pseudomonas sp. strain from used motor oil-contaminated soil[J]. International Biodeterioration & Biodegradation, 2017, 122(8):128-140.
[20] 李康. 盐环境下降解菌群对芘的降解特性研究[J]. 环境污染与防治, 2018, 40(7):755-759.
[21] 宋立超, 刘灵芝, 李培军, 等. 盐碱土壤多环芳烃降解菌群筛选及其降解特性[J]. 石油学报:石油加工, 2012, 28(1):161-166.
[22] Mnif S, Chebbi A, Mhiri N, et al.Biodegradation of phenanthrene by a bacterial consortium enriched from sercina oilfield[J]. Process Safety and Environmental Protection, 2017, 107(4):44-53.
[23] Pugazhendi A, Qari H, Basahi AB, et al.Role of a halothermophilic bacterial consortium for the biodegradation of PAHs and the treatment of petroleum wastewater at extreme conditions[J]. International Biodeterioration & Biodegradation, 2017, 121(7):44-54.
[24] Wang F, Li C, Wang H, et al.Characterization of a phenanthrene-degrading microbial consortium enriched from petrochemical contaminated environment[J]. International Biodeterioration & Biodegradation, 2016, 115(11):286-292.
[25] Patel V, Patel J, Madamwar D.Biodegradation of phenanthrene in bioaugmented microcosm by consortium ASP developed from coastal sediment of alang-sosiya ship breaking yard[J]. Marine Pollution Bulletin, 2013, 74(1):199-207.
[26] Hambrick GA, Delaune RD, Patrick WH.Effect of estuarine sediment pH and oxidation-reduction potential on microbial hydrocarbon degradation[J]. Applied & Environmental Microbiology, 1980, 40(2):365-369.
[27] 王涛, 蓝慧, 田云, 等. 多环芳烃的微生物降解机制研究进展[J]. 化学与生物工程, 2016, 33(2):8-14.
[28] Aydin S, Karacay HA, Shahi A, et al.Aerobic and anaerobic fungal metabolism and omics insights for increasing polycyclic aromatic hydrocarbons biodegradation[J]. Fungal Biology Reviews, 2017, 31(2):61-72.
[29] 王慧, 周海燕, 黄勇, 等. 一株高环多环芳烃降解嗜盐菌Thalassospira sp. 的分离及降解特性[J]. 清华大学学报:自然科学版, 2015, 55(1):87-92.
[30] Rabodonirina S, Rasolomampianina R, Krier F, et al.Degradation of flourene and phenanthrene in PAHs-contaminated soil using pseudomonas and bacillus strains isolated from oil spill sites[J]. Journal of Environmental Management, 2019, 232(15):1-7.
[31] 苏丹, 李培军, 王鑫, 等. 3株细菌对土壤中芘和苯并芘的降解及其动力学[J]. 环境科学, 2007, 28(4):913-917.