Acquisition and Expression Analysis of the Transcription Factor Whirly1 from Haematococcus pluvialis

doi:10.13560/j.cnki.biotech.bull.1985.2020-1348

Abstract

Abstract:

Whirly transcription factors are found in plants ubiquitously,and play a role in plant growth and stress resistance. Uncovering the functions of Whirly transcription factors would help us understand the response mechanism to stress in Haematococcus pluvialis. Hence,a Whirly transcription factor named HpWhirly1 was isolated and cloned based on the transcriptome data of H. pluvialis 192.80 and its structure and expression were analyzed. The cDNA sequence of this transcription factor consisted of 924 bp and genome sequence was 2 556 bp with 5 exons and 4 introns. Results showed that HpWhirly1 was predicted to contain 244 amino acids and had the conserved domain of Whirly transcription factor. Further analysis showed that the expression of HpWhirly1 was up-regulated during the early stage of sodium acetate and salicylic acid stresses,while it was down-regulated under strong light sodium acetate combined stress and nitrogen deficiency stress. The above results indicated that HpWhirly1 might play a role in H. pluvialis when facing stresses and could respond to them. The results of this study are conducive to further studying the functions of Whirly transcription factors and its effects on the stress response of H. pluvialis.

Key words: Haematococcus pluvialis, Whirly, transcription factor, molecular cloning, transcription profile

LIU Wen-fu, HUANG Dan-qiong, HU Qun-ju, WANG Chao-gang. Acquisition and Expression Analysis of the Transcription Factor Whirly1 from Haematococcus pluvialis[J]. Biotechnology Bulletin, 2021, 37(11): 248-256.

Figures/Tables 8

Fig. 1 Cloning of HpWhirly1 gene from Haematococcus pluvialis

Fig. 2 Amino acid sequence of HpWhirly1

Fig.3 Gene structure analysis of HpWhirly1 The orange parts are exons,the blue parts are UTR regions,and the black lines are introns

Fig.4 Conserved domain analysis of HpWhirly1

Fig.5 Predicted 3D structure of HpWhirly1

Fig.6 Homology analysis of HpWhirly1 and other WHYs from different species Haematococcus lacustris（GFH09517.1）,Chlamydomonas eustigma（GAX74163.1）,Dunaliella salina（KAF5835724.1）,and Micractinium conductrix（PSC72124.1）. Three Whirly transcription factor sequences in the model organism Arabidopsis:AtWHY1（AT1G14410）,AtWHY2（AT1G71260）,and AtWHY3（AT2G02740）

Fig.7 Phylogenetic tree analysis of the Whirly transcription factor

Fig.8 Expression analysis of HpWhirly1 under different stress conditions A: Strong light treatment. B: Acetic acid treatment. C: Strong light sodium acetate combined treatment. D: Salicylic acid treatment. E: Nitrogen deficiency treatment. * indicates significant difference between treatment group and control group（P<0.05）. ** indicates extremely significant difference between treatment group and control group（P<0.01）

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