Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (2): 195-202.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0699
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HE Yang1,2(), YU Qiao-ling1, WANG Jun1,2(), QIN Chuan-jie1,2, LI Hua-tao1
Received:
2020-06-05
Online:
2021-02-26
Published:
2021-02-26
Contact:
WANG Jun
E-mail:10001431@njtc.edu.cn;10001407@njtc.edu.cn
HE Yang, YU Qiao-ling, WANG Jun, QIN Chuan-jie, LI Hua-tao. Advances in Prokaryotic Expression Gene of Tilapia[J]. Biotechnology Bulletin, 2021, 37(2): 195-202.
序号 | 基因名称 | 品系 | 载体 | 表达菌株 | 参考文献 |
---|---|---|---|---|---|
1 | 促性腺激素释放激素(GnRH) | 奥利亚罗非鱼 | pMAL-c2x | 大肠杆菌TB1 | [ |
2 | 膜结合型免疫球蛋白D(mIgD) | 吉富罗非鱼 | pET32 a(+) | 大肠杆菌BL21 | [ |
3 | 抗苗勒氏管激素(AMH) | 奥利亚罗非鱼 | pGEX-5x-1 | 大肠杆菌BL21 | [ |
4 | 性腺型芳香化酶基因(cyp19a1a) | 尼罗罗非鱼 | pET-28a | 大肠杆菌BL21 | [ |
5 | 半乳糖凝集素-3(Galectin-3) | 尼罗罗非鱼 | pGEX-4T | 大肠杆菌BL21 | [ |
6 | 热休克蛋白(Hsc70) | 尼罗罗非鱼 | pET-B2m | 大肠杆菌BL21 | [ |
7 | 淋巴细胞抗原 75(Ly75) | 尼罗罗非鱼 | pEASY-E2 | 大肠杆菌BL21(DE3) | [ |
8 | 非特异性细胞毒性细胞受体蛋白(NCCRP- 1) | 尼罗罗非鱼 | pGEX-4T-1 | 大肠杆菌BL21 | [ |
9 | P2X4嘌呤受体(P2X4R) | 尼罗罗非鱼 | pCold II | 大肠杆菌BL21(DE3) | [ |
10 | 过氧化物酶体增殖物激活受体δ(PPARδ) | 尼罗罗非鱼 | pET-B2m | 大肠杆菌BL21 | [ |
11 | 转化生长因子β1(TGF-β1) | 尼罗罗非鱼 | pET-B2m | 大肠杆菌BL21 | [ |
12 | Zeta链相关蛋白-70(ZAP-70) | 尼罗罗非鱼 | pET-B2m | 大肠杆菌BL21 | [ |
13 | 补体3(C3) | 尼罗罗非鱼 | pGEX-4T | 大肠杆菌BL21 | [ |
14 | 髓样分化因子(MyD88) | 尼罗罗非鱼 | pET-B2m | 大肠杆菌BL21 | [ |
15 | 淋巴细胞特异性蛋白酪氨酸激酶(Lck) | 尼罗罗非鱼 | pET-B2m | 大肠杆菌BL21(DE3) | [ |
16 | T淋巴细胞特异分化抗原(CD3/δ、CD4、CD8a、Foxp3) | 尼罗罗非鱼 | PcoldI/pET-28a | 大肠杆菌BL21(DE3) | [ |
17 | 肿瘤坏死因子相关凋亡诱导配体(TNFSF10/TRAIL) | 尼罗罗非鱼 | pET-32a | Rosetta-gami | [ |
18 | 17β-羟类固醇脱氢酶(17β-HSD) | 尼罗罗非鱼 | pET Blue2 | NovaBlue细胞 | [ |
19 | γ-干扰素(IFN-γ) | 尼罗罗非鱼 | PET-30c | 大肠杆菌BL21(DE3) | [ |
20 | 免疫球蛋白M/T(IgM/IgT)重链基因 | 尼罗罗非鱼 | PSUMO(IgM)/PET-28a(IgM、IgT) | 大肠杆菌BL21 | [ |
21 | 半乳糖凝集素-8(Galectin-8) | 尼罗罗非鱼 | pGEX-4T | 大肠杆菌BL21 | [ |
22 | γ-干扰素诱导的溶酶体巯基还原酶(GILT) | 尼罗罗非鱼 | PET28a | 大肠杆菌BL21 | [ |
23 | Doublesex和Mab-3相关的转录因子1(Dmrt1)、抗缪勒氏管激素(Amh)和醛固酮合成酶基因(Cyp11b2) | 尼罗罗非鱼 | pCold I | 大肠杆菌BL21(DE3) | [ |
24 | 铁调素(hepcidin) | 尼罗罗非鱼 | pET-32a | 大肠杆菌BL21(DE3) | [ |
25 | 胰岛素样生长因子3(IGF-3) | 尼罗罗非鱼 | Pcold I和pGex-4T-1 | 大肠杆菌BL21(DE3) | [ |
26 | 翻译延伸因子1A(eEFIA) | 尼罗罗非鱼 | pCold I和pGex-4T-1 | 大肠杆菌Rosetta | [ |
27 | 白血病抑制因子(Lif) | 尼罗罗非鱼 | pET22、pET28a及pSUMO | 大肠杆菌BL21(DE3) | [ |
28 | 白细胞介素1β(IL-1β) | 尼罗罗非鱼 | pQE | 大肠杆菌 | [ |
29 | 罗非鱼催乳素I/II(TiPRL-I or II) | 莫桑比罗非鱼 | pT7 | 大肠杆菌BL21(DE3) | [ |
30 | 罗非鱼催乳素 (TiPRL-I) | 尼罗罗非鱼 | pT7 | 大肠杆菌BL21(DE3) | [ |
31 | 胰岛素样生长因子(IGF-2) | 莫桑比罗非鱼 | pGEX-2T | 大肠杆菌BL21(DE3) | [ |
32 | 胰岛素样生长因子(IGF-2) | 莫桑比罗非鱼 | pET28 | 大肠杆菌BL21(DE3) | [ |
33 | 生长激素(GH) | 尼罗罗非鱼 | pT7 | 大肠杆菌BL21(DE3) | [ |
34 | C型溶菌酶(Lysozyme-C) | 奥利亚罗非鱼 | pQE30 | 大肠杆菌M15 | [ |
序号 | 基因名称 | 品系 | 载体 | 表达菌株 | 参考文献 |
---|---|---|---|---|---|
1 | 促性腺激素释放激素(GnRH) | 奥利亚罗非鱼 | pMAL-c2x | 大肠杆菌TB1 | [ |
2 | 膜结合型免疫球蛋白D(mIgD) | 吉富罗非鱼 | pET32 a(+) | 大肠杆菌BL21 | [ |
3 | 抗苗勒氏管激素(AMH) | 奥利亚罗非鱼 | pGEX-5x-1 | 大肠杆菌BL21 | [ |
4 | 性腺型芳香化酶基因(cyp19a1a) | 尼罗罗非鱼 | pET-28a | 大肠杆菌BL21 | [ |
5 | 半乳糖凝集素-3(Galectin-3) | 尼罗罗非鱼 | pGEX-4T | 大肠杆菌BL21 | [ |
6 | 热休克蛋白(Hsc70) | 尼罗罗非鱼 | pET-B2m | 大肠杆菌BL21 | [ |
7 | 淋巴细胞抗原 75(Ly75) | 尼罗罗非鱼 | pEASY-E2 | 大肠杆菌BL21(DE3) | [ |
8 | 非特异性细胞毒性细胞受体蛋白(NCCRP- 1) | 尼罗罗非鱼 | pGEX-4T-1 | 大肠杆菌BL21 | [ |
9 | P2X4嘌呤受体(P2X4R) | 尼罗罗非鱼 | pCold II | 大肠杆菌BL21(DE3) | [ |
10 | 过氧化物酶体增殖物激活受体δ(PPARδ) | 尼罗罗非鱼 | pET-B2m | 大肠杆菌BL21 | [ |
11 | 转化生长因子β1(TGF-β1) | 尼罗罗非鱼 | pET-B2m | 大肠杆菌BL21 | [ |
12 | Zeta链相关蛋白-70(ZAP-70) | 尼罗罗非鱼 | pET-B2m | 大肠杆菌BL21 | [ |
13 | 补体3(C3) | 尼罗罗非鱼 | pGEX-4T | 大肠杆菌BL21 | [ |
14 | 髓样分化因子(MyD88) | 尼罗罗非鱼 | pET-B2m | 大肠杆菌BL21 | [ |
15 | 淋巴细胞特异性蛋白酪氨酸激酶(Lck) | 尼罗罗非鱼 | pET-B2m | 大肠杆菌BL21(DE3) | [ |
16 | T淋巴细胞特异分化抗原(CD3/δ、CD4、CD8a、Foxp3) | 尼罗罗非鱼 | PcoldI/pET-28a | 大肠杆菌BL21(DE3) | [ |
17 | 肿瘤坏死因子相关凋亡诱导配体(TNFSF10/TRAIL) | 尼罗罗非鱼 | pET-32a | Rosetta-gami | [ |
18 | 17β-羟类固醇脱氢酶(17β-HSD) | 尼罗罗非鱼 | pET Blue2 | NovaBlue细胞 | [ |
19 | γ-干扰素(IFN-γ) | 尼罗罗非鱼 | PET-30c | 大肠杆菌BL21(DE3) | [ |
20 | 免疫球蛋白M/T(IgM/IgT)重链基因 | 尼罗罗非鱼 | PSUMO(IgM)/PET-28a(IgM、IgT) | 大肠杆菌BL21 | [ |
21 | 半乳糖凝集素-8(Galectin-8) | 尼罗罗非鱼 | pGEX-4T | 大肠杆菌BL21 | [ |
22 | γ-干扰素诱导的溶酶体巯基还原酶(GILT) | 尼罗罗非鱼 | PET28a | 大肠杆菌BL21 | [ |
23 | Doublesex和Mab-3相关的转录因子1(Dmrt1)、抗缪勒氏管激素(Amh)和醛固酮合成酶基因(Cyp11b2) | 尼罗罗非鱼 | pCold I | 大肠杆菌BL21(DE3) | [ |
24 | 铁调素(hepcidin) | 尼罗罗非鱼 | pET-32a | 大肠杆菌BL21(DE3) | [ |
25 | 胰岛素样生长因子3(IGF-3) | 尼罗罗非鱼 | Pcold I和pGex-4T-1 | 大肠杆菌BL21(DE3) | [ |
26 | 翻译延伸因子1A(eEFIA) | 尼罗罗非鱼 | pCold I和pGex-4T-1 | 大肠杆菌Rosetta | [ |
27 | 白血病抑制因子(Lif) | 尼罗罗非鱼 | pET22、pET28a及pSUMO | 大肠杆菌BL21(DE3) | [ |
28 | 白细胞介素1β(IL-1β) | 尼罗罗非鱼 | pQE | 大肠杆菌 | [ |
29 | 罗非鱼催乳素I/II(TiPRL-I or II) | 莫桑比罗非鱼 | pT7 | 大肠杆菌BL21(DE3) | [ |
30 | 罗非鱼催乳素 (TiPRL-I) | 尼罗罗非鱼 | pT7 | 大肠杆菌BL21(DE3) | [ |
31 | 胰岛素样生长因子(IGF-2) | 莫桑比罗非鱼 | pGEX-2T | 大肠杆菌BL21(DE3) | [ |
32 | 胰岛素样生长因子(IGF-2) | 莫桑比罗非鱼 | pET28 | 大肠杆菌BL21(DE3) | [ |
33 | 生长激素(GH) | 尼罗罗非鱼 | pT7 | 大肠杆菌BL21(DE3) | [ |
34 | C型溶菌酶(Lysozyme-C) | 奥利亚罗非鱼 | pQE30 | 大肠杆菌M15 | [ |
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