Screening and Identification of Highly Efficient Crude Oil-degrading Bacteria and Construction of Bacterial Consortium

doi:10.13560/j.cnki.biotech.bull.1985.2020-1094

Abstract

Abstract:

In order to obtain efficient crude oil-degrading strains,9 strains of microorganisms were isolated and screened from the oily sludge of an oil field in Shandong,laboratory’s own bacterial agents and crude oil contaminated soil,which were numbered SF1,SF2,SF3,SF4,SF5,SF6,SF7,SF8 and SF9. Studies have shown that 9 strains presented certain abilities of degrading crude oil. Via 16S rRNA identification,SF1 belonged to Ochrobactrum intermedium,SF2 and SF8 belonged to Enterobacter sp,SF3 and SF7 belonged to Acinetobacter sp,SF4,SF5,SF6 and SF9 belonged to Pseudomonas sp. Among them,strain SF1 has the strongest degradability,with a degradation rate of 48.73%,strains SF2,SF7,and SF9 showed strong degradability,with degradation rates of 41.90%,45.35%,and 32.25%,respectively. The degradation rates of other strains were higher than 20%. Through orthogonal experiments,it is concluded that the bacterial flora composed of strains SF1,SF2,SF3,SF4,SF5,SF6,SF7and SF9 with the addition amount of 2∶1∶1∶1∶1∶1∶2∶2 has an effect on crude oil,and the degradation effect is better than that by a single strain,and this flora is the best efficient one,with a degradation rate of 57.88%.

Key words: crude oil pollution, highly efficient degrading bacteria, screening, identification, flora construction

ZHU Shu-fang, EHENEDEN Iyobosa, NING Hai-jun, SHANG Jie-hao, LI Wu-yang, MENG Xian-gang. Screening and Identification of Highly Efficient Crude Oil-degrading Bacteria and Construction of Bacterial Consortium[J]. Biotechnology Bulletin, 2021, 37(4): 107-115.

Figures/Tables 6

References 33

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实验组Experimental group	SF1	SF2	SF3	SF4	SF5	SF6	SF7	SF8	SF9	降解率Degradation rate/%
1	0	0	1	0.5	0.5	0	0.5	0	0	30.20
2	1	0.5	1	0	0	0.5	0.5	0	0	38.00
3	0.5	0.5	1	0.5	0	0	1	0.5	0.5	41.25
4	0.5	0.5	0.5	1	0.5	0	0	0.5	0	43.60
5	1	0	0.5	0	1	1	0	0.5	1	37.10
6	0.5	1	0.5	0	0	1	1	0	0	31.1
7	0	1	0.5	0.5	0	0.5	0	0.5	0.5	34.45
8	0.5	0	0.5	0.5	1	0.5	0.5	1	0	28.60
9	1	0.5	0.5	0.5	0.5	0.5	1	0	1	57.88
10	1	1	1	0.5	1	0	0	1	0	24.90
11	0.5	0	0	1	0	0.5	1	1	1	28.55
12	0	0.5	0	0.5	1	1	1	1	0	40.90
13	0	1	0	1	0.5	0.5	0.5	0.5	0	19.85
14	1	1	0	0	0.5	0	1	1	0.5	28.75
15	0	0	0.5	1	1	0	1	0	0.5	30.05
16	0.5	0	1	0	0.5	0.5	0	1	0.5	19.30
17	0	0.5	1	1	0	1	0	1	1	25.80
18	0	0	0	0	0	0	0	0	0	2.50
19	1	0	0	0.5	0	1	0.5	0.5	0.5	31.60
20	0	1	1	0	1	0.5	1	0.5	1	27.30
21	1	0.5	0	1	1	0.5	0	0	0.5	11.10
22	0.5	1	0	0.5	0.5	1	0	0	1	34.05
23	0	0.5	0.5	0	0.5	1	0.5	1	0.5	17.35
24	1	1	0.5	1	0	0	0.5	1	1	28.65
25	0.5	1	1	1	1	1	0.5	0	0.5	14.35
26	1	0	1	1	0.5	1	1	0.5	0	7.60
27	0.5	0.5	0	0	1	0	0.5	0.5	1	23.80

实验组Experimental group	SF1	SF2	SF3	SF4	SF5	SF6	SF7	SF8	SF9	降解率Degradation rate/%
1	0	0	1	0.5	0.5	0	0.5	0	0	30.20
2	1	0.5	1	0	0	0.5	0.5	0	0	38.00
3	0.5	0.5	1	0.5	0	0	1	0.5	0.5	41.25
4	0.5	0.5	0.5	1	0.5	0	0	0.5	0	43.60
5	1	0	0.5	0	1	1	0	0.5	1	37.10
6	0.5	1	0.5	0	0	1	1	0	0	31.1
7	0	1	0.5	0.5	0	0.5	0	0.5	0.5	34.45
8	0.5	0	0.5	0.5	1	0.5	0.5	1	0	28.60
9	1	0.5	0.5	0.5	0.5	0.5	1	0	1	57.88
10	1	1	1	0.5	1	0	0	1	0	24.90
11	0.5	0	0	1	0	0.5	1	1	1	28.55
12	0	0.5	0	0.5	1	1	1	1	0	40.90
13	0	1	0	1	0.5	0.5	0.5	0.5	0	19.85
14	1	1	0	0	0.5	0	1	1	0.5	28.75
15	0	0	0.5	1	1	0	1	0	0.5	30.05
16	0.5	0	1	0	0.5	0.5	0	1	0.5	19.30
17	0	0.5	1	1	0	1	0	1	1	25.80
18	0	0	0	0	0	0	0	0	0	2.50
19	1	0	0	0.5	0	1	0.5	0.5	0.5	31.60
20	0	1	1	0	1	0.5	1	0.5	1	27.30
21	1	0.5	0	1	1	0.5	0	0	0.5	11.10
22	0.5	1	0	0.5	0.5	1	0	0	1	34.05
23	0	0.5	0.5	0	0.5	1	0.5	1	0.5	17.35
24	1	1	0.5	1	0	0	0.5	1	1	28.65
25	0.5	1	1	1	1	1	0.5	0	0.5	14.35
26	1	0	1	1	0.5	1	1	0.5	0	7.60
27	0.5	0.5	0	0	1	0	0.5	0.5	1	23.80