Physiological and Biochemical Characteristics of Inquilinus sp. P6-4 Strain and Its Degradation Characteristics for Naphthalene

doi:10.13560/j.cnki.biotech.bull.1985.2020-1392

Abstract

Abstract:

The P6-4 strain screened from tailings was taken as the research object,its taxonomic status was determined based on 16S rDNA sequencing,and its physiological and biochemical characteristics and degradation characteristics for naphthalene were studied. Concurrently,the optimal degradation conditions and degradation pathways of the strain for naphthalene were analyzed. The results showed that P6-4 strain was a gram-negative aerobic bacterium of the genus Inquilinus,grew on R₂A medium with a salinity of 1%-3% and citrate medium,and produced catalase,IAA and iron carriers. It had the function of dissolving phosphorus and fixing nitrogen. It did not produce amylase and acetyl methyl methanol while decomposing glucose. The strain grew the best under the conditions of initial naphthalene mass concentration of 400 mg/L,30℃,pH 9.0,and 150 r/min. After 7 d of culture under the optimal degradation conditions,using n-hexane as the extractant,the degradation rate measured by UV-V is spectrophotometry was 93.22%. The study of molecular biology revealed that the strain contained PAH(PAH-ring hydroxylating dioxygenase gene),HBHA(trans-o-hydroxybenzylidenepyruvate hydratase-aldolase gene),nahU(salicylate hydroxylase gene),S5H(salicylate 5-hydroxylase gene),CATA(catechol 1,2-dioxygenase gene),C230(catechol 2,3-dioxygenase gene),and GDO(gentisate 1,2-dioxygenase gene),and it was speculated that the P6-4 strain degraded naphthalene in two pathways:salicylic acid and and gentianic acid.

Key words: Inquilinus sp., naphthalene, physiological and biochemical characteristics, degradation characteristics

WANG Ying, CHEN Yong-jing, SUN Qing-ye, YANG Meng-yao, WU Dun. Physiological and Biochemical Characteristics of Inquilinus sp. P6-4 Strain and Its Degradation Characteristics for Naphthalene[J]. Biotechnology Bulletin, 2021, 37(6): 117-126.

Figures/Tables 7

References 54

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生化反应 Physiological and biochemical reaction	结果 Result	生化反应 Physiological and biochemical reaction	结果 Result
1%、2%、3% NaCl生长 1%,2%,3% NaCl growth	+	固氮 Nitrogen fixation	+
过氧化氢酶 Catalase	+	柠檬酸盐 Utilization of citrate	+
吲哚乙酸 Indole acetic acid	+	VP试验 VP test	-
有机磷 Organophosphorus	+	厌氧生长 Anaerobic growth	-
无机磷 Inorganic phosphorus	+	淀粉酶 Amylase	-
革兰氏染色 Gram staining	-	铁载体 Iron carrier	+

生化反应 Physiological and biochemical reaction	结果 Result	生化反应 Physiological and biochemical reaction	结果 Result
1%、2%、3% NaCl生长 1%,2%,3% NaCl growth	+	固氮 Nitrogen fixation	+
过氧化氢酶 Catalase	+	柠檬酸盐 Utilization of citrate	+
吲哚乙酸 Indole acetic acid	+	VP试验 VP test	-
有机磷 Organophosphorus	+	厌氧生长 Anaerobic growth	-
无机磷 Inorganic phosphorus	+	淀粉酶 Amylase	-
革兰氏染色 Gram staining	-	铁载体 Iron carrier	+