Effects of Crude Polysaccharide from Cistanche deserticola in Xinjiang on Foot-and-Mouth Disease Viral Vaccine Antibody and T cell Subgroup

doi:10.13560/j.cnki.biotech.bull.1985.2020-1455

Abstract

Abstract:

To screen new polysaccharide vaccine adjuvants of foot-and-mouth disease,the crude polysaccharide from Cistanche deserticola in Xinjiang（CDCP）and inactivated foot-and-mouth disease viral antigen（FMDV-Ag）were selected to prepare water adjuvant vaccines,ISA-206 oil adjuvant vaccine and adjuvant-free vaccine. ICR mice were immunized by muscle. Serum antibody level,lymphocyte proliferation response,and expressions of CD3⁺CD4⁺,CD3⁺CD8⁺ and CD4⁺CD44⁺,CD8⁺CD44⁺T cell subsets were measured to evaluate the immune enhancement effect after the vaccination,and the behaviors and weights of the mice were monitored for observing the safety of the vaccines. The results showed CDCP substantially enhanced the FMDV-specific antibody level（P<0.05）and significantly increased lymphocyte proliferative responses（P<0.05）. T responses were also significantly enhanced（P<0.05）,including proliferative responses of CD4⁺,CD8⁺,and CD44⁺ T cells. No abnormal behavior for mice after immunization was observed and the weights of mice also were not affected. CDCP as water adjuvant of FMDV-Ag has a fine immune-promoting effect by enhancing humoral and cellular immune response.

Key words: Cistanche deserticola in Xinjiang, crude polysaccharide, foot-and-mouth disease viral vaccine, adjuvant

ZHANG Ai-lian, BA Xue-li, WANG Dan-yang, ZHAO Bing. Effects of Crude Polysaccharide from Cistanche deserticola in Xinjiang on Foot-and-Mouth Disease Viral Vaccine Antibody and T cell Subgroup[J]. Biotechnology Bulletin, 2021, 37(9): 212-218.

Figures/Tables 5

Fig.1 Detection on FMDV-specific antibody by indirect ELISA Mice were intramuscularly immunized with CDCP and FMDV-Ag,on 14,28 and 42 d after primary immunization,sera were collected. The level of FMDV-specific antibody was detected by ELISA（n=6）. *P < 0.05,**P < 0.01 and ***P < 0.001 compared with FMDV-Ag group. The same below

Fig. 2 Effect of CDCP on splenocyte proliferation Mice were intramuscularly immunized with CDCP and FMDV-Ag,splenocyte suspension was isolated on 21 d after primary immunization,and the effects of CPCD on splenocyte proliferation were determined by MTT assay（n=6）

Fig.3 Effects of CDCP on CD4+ and CD8+ T cell subsets Mice were immunized with CDCP and FMDV-Ag on 21 d after primary immunization,cells from spleen of mice were used to detect T cell subsets by flow cytometry（n=6）

Fig.4 Effects of CDCP on CD4+ CD44+ and CD8+ CD44+ effector T cell On 21 d after primary immunization,flow cytometry was used to detect the effects of CDCP on CD4+CD44+ and CD8+CD44+ effector T cell in spleen（n=6）

Table 1 Changes in body weight of mice immunized with FMDV/CDCP

组别 Group	每组小鼠数 Number of each group（n）	体重 Weight/g
组别 Group	每组小鼠数 Number of each group（n）	0 d	14 d	21 d	28 d
0.9%NaCl	5	21.60±1.01	27.20±2.52	31.72±2.85	32.51±3.01
FMDV-Ag	5	23.22±1.21	28.68±2.34	32.54±1.87	34.18±2.72
FMDV-Ag/CDCP	5	22.68±0.37	27.26±2.28	31.72±2.55	34.44±2.73
FMDV/ISA-206	5	21.38±1.04	27.58±1.44	30.56±1.28	32.96±3.01

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