Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (3): 22-30.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0797

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Cloning and Expression Analysis of CsCML24 Gene in Camellia sinensis

KANG Rui(), LIU Chun-hui, CHEN Si-wen, ZHAO Ren-liang, ZHOU Qiong-qiong()   

  1. College of Horticulture,Henan Agricultural University,Zhengzhou 450000
  • Received:2021-06-23 Online:2022-03-26 Published:2022-04-06
  • Contact: ZHOU Qiong-qiong E-mail:1350310379@qq.com;zqxy1223@henau.edu.cn

Abstract:

Calmodulin-like protein(CML)is a type of calcium receptor protein,which mediates the interaction of Ca2+ with downstream target proteins,and plays an important role in plant abiotic stress response. Exploring the function of CML in tea plant(Camellia sinensis) under adversity stress could provide theoretical references for further studying the molecular regulation mechanism of CsCML24 in stress response of tea plants. The CsCML24 gene(GenBank accession number:MZ325391)of CML was cloned from the leaves of tea cultivar ‘Longjing 43’ and analyzed by bioinformatics. Meanwhile,the relative tissue-specific expressions and expression patterns under different abiotic stresses were analyzed by real-time quantitative PCR. Results of sequence analysis indicated that the full-length CDS sequence of the CsCML24 gene was 480 bp,encoding 159 amino acids. The CsCML24 had no signal peptide and transmembrane region,and belonged to hydrophilic protein,contained EF-hands,which can be combined with Ca2+. Tissue-specific expression results from qRT-PCR showed that the CsCML24 gene expressed in various tissues,and highly expressed in the mature leaves,and the least in the stem. The expression of CsCML24 was significantly induced under low temperature(10℃),drought stress(20% PEG 6000),salt stress(200 mmol/L NaCl)and ABA,and the expression varied in different stress. It is speculated that this gene regulates the low temperature and drought tolerance of plants via ABA signaling pathway.

Key words: Camellia sinensis, calmodulin-like protein, abiotic stress, expression analysis