Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (6): 136-146.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1124
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ZU Xue1,2,3(), ZHOU Hu1,2,3, ZHU Hua-jun1,2,3, REN Zuo-hua1,2,3, LIU Er-ming1,2,3()
Received:
2021-09-01
Online:
2022-06-26
Published:
2022-07-11
Contact:
LIU Er-ming
E-mail:1762823097@qq.com;ermingliu@163.com
ZU Xue, ZHOU Hu, ZHU Hua-jun, REN Zuo-hua, LIU Er-ming. Isolation and Identification of Bacillus subtilis K-268 and Its Biological Control Effect on Rice Blast[J]. Biotechnology Bulletin, 2022, 38(6): 136-146.
病原菌Pathogenic bacterium | 抑菌率Bacteriostatic rate/% |
---|---|
水稻纹枯病菌R. solan | 79.5±0.67 |
玉米大斑菌E.turcicum | 47.6±0.33 |
柑橘沙皮病菌D. citri | 21±5.7 |
草莓灰霉病菌 B. cinerea | 49.6±1.8 |
茭白根腐病菌F. graminearum | 43.5±10.3 |
辣椒枯萎病菌F. oxysporum | 62±4.0 |
茄子根腐病菌F. solani | 61.2±10.3 |
香樟炭疽病菌C. gloeosporioides | 32.8±5 |
棉花黄萎病菌 V. dahliae | 36.8±3.38 |
禾谷镰刀菌F.graminearum | 56.7±0.33 |
烟草赤星病菌A. alternate | 49.2±3.1 |
油菜菌核病菌S. sclerotiorum | 56.3±2.33 |
黄瓜疫霉病菌P. melonis | 56.8±0.0 |
水稻恶苗病菌F. moniliforme | 69.2±0.88 |
Table 1 Bacteriostatic effects of strain K-268 on different plant pathogens
病原菌Pathogenic bacterium | 抑菌率Bacteriostatic rate/% |
---|---|
水稻纹枯病菌R. solan | 79.5±0.67 |
玉米大斑菌E.turcicum | 47.6±0.33 |
柑橘沙皮病菌D. citri | 21±5.7 |
草莓灰霉病菌 B. cinerea | 49.6±1.8 |
茭白根腐病菌F. graminearum | 43.5±10.3 |
辣椒枯萎病菌F. oxysporum | 62±4.0 |
茄子根腐病菌F. solani | 61.2±10.3 |
香樟炭疽病菌C. gloeosporioides | 32.8±5 |
棉花黄萎病菌 V. dahliae | 36.8±3.38 |
禾谷镰刀菌F.graminearum | 56.7±0.33 |
烟草赤星病菌A. alternate | 49.2±3.1 |
油菜菌核病菌S. sclerotiorum | 56.3±2.33 |
黄瓜疫霉病菌P. melonis | 56.8±0.0 |
水稻恶苗病菌F. moniliforme | 69.2±0.88 |
生理生化试验Physiological and biochemical tests | 结果Result |
---|---|
接触酶试验Contact enzyme test | + |
硝酸盐还原试验Nitrate reduction test | + |
明胶液化试验Gelatin liquefaction test | + |
硫化氢试验Hydrogen sulfide test | - |
淀粉水解试验Starch hydrolysis test | - |
吲哚试验Indole test | + |
V.P试验V. P test | + |
甲基红试验Methyl red test | - |
石蕊牛奶试验Litmus milk test | 胨化Peptonization |
苯丙氨酸脱氨酶试验Phenylalanine deaminase test | - |
Table 2 Physiological and biochemical characteristics of strain K-268
生理生化试验Physiological and biochemical tests | 结果Result |
---|---|
接触酶试验Contact enzyme test | + |
硝酸盐还原试验Nitrate reduction test | + |
明胶液化试验Gelatin liquefaction test | + |
硫化氢试验Hydrogen sulfide test | - |
淀粉水解试验Starch hydrolysis test | - |
吲哚试验Indole test | + |
V.P试验V. P test | + |
甲基红试验Methyl red test | - |
石蕊牛奶试验Litmus milk test | 胨化Peptonization |
苯丙氨酸脱氨酶试验Phenylalanine deaminase test | - |
Fig. 6 Control effect of strain K-268 on leaf blast(indoor detached-leaf inoculation) a,g:water;b,h:NB;c,i:75% tricyclazole(WP);the concentration of d-f and j-l:6×107 CFU/mL,6×108 CFU/mL and 6×109 CFU/mL
处理Treatment | 发病率Disease incidence(-24 h) | 发病率Disease incidence(+24 h) |
---|---|---|
清水对照CK Water control CK | 98.77±1.23a | 97±2.47a |
NB | 95.06±1.24ab | 98.71±1.23a |
75%三环唑(WP) 75% tricyclazole(WP) | 12.34±1.23d | 17.28±1.24d |
6×109CFU/mL | 93.83±1.24ab | 97.53±2.47a |
6×108CFU/mL | 14.81±2.41d | 23.46±1.24cd |
6×107 CFU/mL | 22.22±2.14c | 27.16±2.47c |
Table 3 In vitro antagonistic effects of different concent-rations of K-268 fermentation broth on rice blast
处理Treatment | 发病率Disease incidence(-24 h) | 发病率Disease incidence(+24 h) |
---|---|---|
清水对照CK Water control CK | 98.77±1.23a | 97±2.47a |
NB | 95.06±1.24ab | 98.71±1.23a |
75%三环唑(WP) 75% tricyclazole(WP) | 12.34±1.23d | 17.28±1.24d |
6×109CFU/mL | 93.83±1.24ab | 97.53±2.47a |
6×108CFU/mL | 14.81±2.41d | 23.46±1.24cd |
6×107 CFU/mL | 22.22±2.14c | 27.16±2.47c |
Fig.7 Partial view of the control effect of strain K-268 agai-nst leaf blast(indoor live inoculation) m,t:water;n,u:NB;the concentrations of o-q and v-x:6×109 CFU/mL,6×108 CFU/mL and 6×107 CFU/mL;r,y:75% tricyclazole(WP);s,z:40% rice pyrazole(EC)
处理 Treatment | 预防组Prevention group | 治疗组Treatment group | ||||||
---|---|---|---|---|---|---|---|---|
病株率Disease rate /% | 病情指数 Disease index | 相对防效Relative control effect /% | 病株率Disease rate/% | 病情指数Disease index | 相对防效Relative control effect/% | |||
水Water | 96.67±0.33a | 61±1.5a | - | 92±1.0a | 56.13±0.52a | - | ||
NB | 90.33±0.88b | 51.6±0.6b | - | 91±0.88a | 54±0.52a | - | ||
6×109 CFU/mL | 66.33±2.18d | 33± 1.5c | 45.7±3.25d | 73±1.33 b | 33.47±1.07 b | 45.5±1.54d | ||
6×108 CFU/mL | 62.33±1.45e | 25±1.5 ef | 59.0±2.80ab | 58.33±1.86e | 22.27±0.65 e | 60.23±0.71b | ||
6×107 CFU/mL | 71.66±1.45c | 34±1.66 c | 38.8±4.89d | 67.33±1.76 c | 34.27±1.18b | 38.9±1.97e | ||
75%三环唑(WP) 75% Tricyclazole(WP) | 53.33±0.88f | 23±1.2 f | 60.6±1.15a | 50.33±0.88 f | 20±1.15e | 67.2±1.94a | ||
40%稻瘟灵(EC) 40% Pyrimethamine(EC) | 67±1.15 d | 28±0.87 e | 53.3±1.50b | 68.77±1.75d | 27.37±0.58d | 51±1. 11c |
Table 4 Control effect of strain K-268 on rice leaf blast in pot
处理 Treatment | 预防组Prevention group | 治疗组Treatment group | ||||||
---|---|---|---|---|---|---|---|---|
病株率Disease rate /% | 病情指数 Disease index | 相对防效Relative control effect /% | 病株率Disease rate/% | 病情指数Disease index | 相对防效Relative control effect/% | |||
水Water | 96.67±0.33a | 61±1.5a | - | 92±1.0a | 56.13±0.52a | - | ||
NB | 90.33±0.88b | 51.6±0.6b | - | 91±0.88a | 54±0.52a | - | ||
6×109 CFU/mL | 66.33±2.18d | 33± 1.5c | 45.7±3.25d | 73±1.33 b | 33.47±1.07 b | 45.5±1.54d | ||
6×108 CFU/mL | 62.33±1.45e | 25±1.5 ef | 59.0±2.80ab | 58.33±1.86e | 22.27±0.65 e | 60.23±0.71b | ||
6×107 CFU/mL | 71.66±1.45c | 34±1.66 c | 38.8±4.89d | 67.33±1.76 c | 34.27±1.18b | 38.9±1.97e | ||
75%三环唑(WP) 75% Tricyclazole(WP) | 53.33±0.88f | 23±1.2 f | 60.6±1.15a | 50.33±0.88 f | 20±1.15e | 67.2±1.94a | ||
40%稻瘟灵(EC) 40% Pyrimethamine(EC) | 67±1.15 d | 28±0.87 e | 53.3±1.50b | 68.77±1.75d | 27.37±0.58d | 51±1. 11c |
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