Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (8): 118-126.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1192

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Research on Mechanism and QTL Mapping Associated with Cadmium Accumulation in Rice Leaves and Grains

HUANG Jing1(), ZHU Liang1,2, XUE Peng-bo1,2, FU Qiang1()   

  1. 1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Guangxi University,Nanning 530004
    2. College of Life Science and Technology,Guangxi University,Nanning 530004
  • Received:2021-09-15 Online:2022-08-26 Published:2022-09-14
  • Contact: FU Qiang E-mail:jhuang@gxu.edu.cn;gxfuq@gxu.edu.cn

Abstract:

Rice is the most important staple food in China,thus mining the key genes controlling cadmium(Cd)accumulation in rice leaves and grains and clarifying the genetic mechanism of Cd accumulation and transport may provide the basis for breeding rice varieties with low cadmium accumulation and ensuring food security and human health. Inomics screening was performed to systemically profile ionic spectrum in the grains of 209 rice varieties from the Rice Core Germplasm cultivated in China. The accessions with differential cadmium and/or iron(Fe)/zinc(Zn)accumulations were identified,from which indicia cultivar HQ03 and japonica cultivar SKC were selected for further study. Results showed that there was no significant difference in cadmium tolerance and uptake between the two varieties,and Cd accumulations in the HQ03 shoots and grains were significantly higher than those in SKC,while no difference in Fe,Zn,etc. Using a doubled haploid(DH)population derived from HQ03 and SKC of 137 individuals,8 quantitative trait loci(QTL)controlling Cd accumulation in the leaves and grains were identified on chromosome 2,3,4,7,8,10 and 11,accounting for 10.6%-39.4% of the phenotypic variance detected. Among them,qGCd3,controlling Cd transport to grains,was delimited in the interval of RM6266-RM2334 on chromosome 3,with an LOD(limit of detection)value of 3.81 and a phenotypic contribution of 39.4%,indicating that an important gene controlling cadmium transport to grain may exist here.

Key words: rice, core germplasm, cadmium, QTL mapping