Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (8): 110-117.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0414
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GUO Zhi-hao1,2(), JIN Ze-xin1,2, LIU Qi1(), GAO Li2()
Received:
2021-04-03
Online:
2022-08-26
Published:
2022-09-14
Contact:
LIU Qi,GAO Li
E-mail:1324254496@qq.com;liuqi@xjau.edu.cn;lgao@ippcaas.cn
GUO Zhi-hao, JIN Ze-xin, LIU Qi, GAO Li. Bioinformatics Analysis, Subcellular Localization and Toxicity Verification of Effector g11335 in Tilletia contraversa Kühn[J]. Biotechnology Bulletin, 2022, 38(8): 110-117.
引物名称 Primer name | 引物序列 Primer sequence(5'-3') |
---|---|
g11335-F | TCAGCACCAGCTAGCATCGATATGGCGCGCCAAC- TCGGCAAC |
g11335-R | AACCGTTCATCGGCGGTCGACTCAAGCGGTTCCA- TCGATCGACA |
g11335-YF | ATTTACGAACGATAGGGTACCATGGCGCGCCAAC- TCGGCAAC |
g11335-YR | GCCCTTGCTCACCATGGATCCTCAAGCGGTTCCA- TCGATCGACA |
pBin-F | ACAATCCCACTATCCTTCGC |
pBin-R | AAGTCGTGCTGCTTCATGT |
PGR107-F | ATAGCAGTCATTAGCACTTCCT |
PGR107-R | CACTGGGGCATACTTCATGC |
Table 1 List of primers
引物名称 Primer name | 引物序列 Primer sequence(5'-3') |
---|---|
g11335-F | TCAGCACCAGCTAGCATCGATATGGCGCGCCAAC- TCGGCAAC |
g11335-R | AACCGTTCATCGGCGGTCGACTCAAGCGGTTCCA- TCGATCGACA |
g11335-YF | ATTTACGAACGATAGGGTACCATGGCGCGCCAAC- TCGGCAAC |
g11335-YR | GCCCTTGCTCACCATGGATCCTCAAGCGGTTCCA- TCGATCGACA |
pBin-F | ACAATCCCACTATCCTTCGC |
pBin-R | AAGTCGTGCTGCTTCATGT |
PGR107-F | ATAGCAGTCATTAGCACTTCCT |
PGR107-R | CACTGGGGCATACTTCATGC |
Fig. 8 Subcellular localization of effector g11335 A: Empty carrier in the GFP channels. B: Empty carrier in the bright field of vision. C: Empty carrier in the compound field. D: g11335 in the GFP channels. E: g11335 in the bright field of vision. F: g11335 in the compound field(Scale: 20 μm)
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