Identification and Drug Resistance of Escherichia coli Simultaneously Producing Carbapenemase NDM-1 and NDM-5

doi:10.13560/j.cnki.biotech.bull.1985.2022-0073

Abstract

Abstract:

In order to understand the prevalence of carbapenemase-carrying NDM-1 and its subtypes in pig farms,87 strains of Escherichia coli were isolated and identified from piglet diarrhea cases. Carbapenemase-resistant genes NDM-1 and NDM-5 were amplified and sequenced. An E. coli strain carrying both NDM-1 and NDM-5 was identified from 87 strains of E. coli and named HN2106. In order to further understand the whole genome of HN2106 and the plasmids and drug-resistant genes carried by HN2106,16S rRNA strain identification of NH2106 was conducted,bla_NDM drug-resistant gene was confirmed,and whole genome sequencing was performed. BLAST and MEGA software were used for bioinformatics analysis and phylogenetic tree analysis. The results showed that the strain contained 7 plasmids（plasmid A,plasmid B,plasmid C,plasmid D,plasmid E,plasmid F,and plasmid G）,in 3 types,were as Col,IncFII,and IncQ1. The strain simultaneously carried gene NDM-1 and NDM-5,they belonged to a β -lactamase of group B,in which bla_NDM-1 was located at plasmid pNDM5-HN2106（plasmid C）,bla_NDM-5 was located at plasmid pNDM5-HN2106（plasmid B）. They mediated bla_NDM-1 and bla_NDM-5 resistance genes,respectively,and were important vectors for the transmission of drug-resistant bacteria. The prediction of whole genome resistance gene demonstrated that HN2106 also carried 21 types of antibiotic resistance genes. The resistances of HN2106 strain to 85 antibiotics were tested,and the results showed that HN2106 was resistant to imipenem,penicillin G,et al.,as well as it was sensitive to sulfamethoxazole,cefmetazole,et al. The above results reveal that NDM-1 drug-resistant plasmid is an important vector of NDM-1 and its subtype resistance genes,which may bring great hidden dangers and threats to healthy breeding and public health safety in the breeding industry. In order to provide theoretical reference for the prevention and treatment of clinical drug-resistant bacterial infection,it is necessary to strengthen the rational application of antibacterial drugs and the prevention and control measures of infection,and pay more attentions to the monitoring of bacterial resistances.

Key words: carbapenase, NDM-1, NDM-5, Escherichia coli, bacterial drug resistance

LI Hai-li, LANG Li-min, ZHANG Qing-xian, YOU Yi, ZHU Wen-hao, WANG Zhi-fang, ZHANG Li-xian, WANG Ke-ling. Identification and Drug Resistance of Escherichia coli Simultaneously Producing Carbapenemase NDM-1 and NDM-5[J]. Biotechnology Bulletin, 2022, 38(9): 106-115.

Figures/Tables 6

References 53

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引物Primers name	引物序列Primer sequence（5'-3'）	产物长度Amplicon size/bp	退火温度PCR annealing temperature/℃
NDM-5F	ATGGAATTGCCCAATATTATGCAC	480	58
NDM-5R	TCAGCGCAGCTTGTCGGC	480	58
NDM-1F	GGTTTGGCGATCTGGTTTTC	593	57
NDM-1R	CGGAATGGCTCATCACGATC	593	57

引物Primers name	引物序列Primer sequence（5'-3'）	产物长度Amplicon size/bp	退火温度PCR annealing temperature/℃
NDM-5F	ATGGAATTGCCCAATATTATGCAC	480	58
NDM-5R	TCAGCGCAGCTTGTCGGC	480	58
NDM-1F	GGTTTGGCGATCTGGTTTTC	593	57
NDM-1R	CGGAATGGCTCATCACGATC	593	57

抗生素Antibiotics	HN2106	抗生素Antibiotics	HN2106	抗生素Antibiotics	HN2106	抗生素Antibiotics	HN2106
亚胺培南Imipenem	R	头孢西丁Cefoxitin	R	庆大霉素Gentamicin	R	奈替米星Netilmicin	R
氨苄西林Ampicillin	R	头孢克洛Cefaclor	R	卡那霉素Kanamycin	R	诺氟沙星Norfloxacin	R
海他西林Hetacillin	R	头孢呋辛Cefuroxime	R	阿米卡星Amikacin	R	新霉素Neomycin	R
阿莫西林 Amoxicillin	R	头孢噻肟 Cefotaxime	R	链霉素 Streptomycin	R	羧苄西林 Carbenicillin	R
苯唑西林Oxacillin	R	头孢唑肟ceftizoxime	R	多西环素Doxycycline	R	哌拉西林Piperacillin	R
氯唑西林 Cloxacillin	R	头孢曲松 Ceftriaxone	R	四环素 Tetracycline	R	妥布霉素 Tobramycin	R
美洛培南 Meropenem	R	头孢哌酮 Cefoperazone	R	氯霉素 Chloramphenicol	R	氟罗沙星 Fleroxacin	R
多黏菌素B Polymyxin B	R	头孢他啶 Ceftazidime	R	克林霉素 Clindamycin	R	头孢他美 Cefetamet	R
美洛西林/舒巴坦 Mezlocillin/Sulbactam	R	头孢噻呋 Ceftiofur	R	红霉素 Erythromycin	R	万古霉素 Vancomycin	R
阿莫西林/舒巴坦 Amoxicillin/sulbactam	R	头孢比肟 Cefepime	R	头孢羟氨苄 Cefadroxil	R	利福平 Rifampicin	R
阿莫西林/克拉维酸 Amoxicillin/clavulanic acid	R	头孢喹肟 Cefquinome	R	呋喃妥因 Nitrofurantoin	R	头孢地嗪 Cefodizime	R
磺胺甲异恶唑 Sulfamethoxazole	R	左氧氟沙星 Levofloxacin	R	头孢匹胺 Cefpiramide	R	头孢噻吩 Cephalothin	R
氨苄西林/舒巴坦 Ampicillin/Sulbactam	R	司帕沙星 Sparfloxacin	R	头孢克肟 Cefixime	R	依替米星 Etimicin	R
磺胺甲恶唑 Sulfamethoxazole	S	美洛西林 Mezlocillin	R	罗红霉素 Roxithromycin	R	替考拉宁 Teicoplanin	R
头孢美唑Cefmetazole	S	庆大霉素Gentamicin	R	加替沙星Gatifloxacin	R	磷霉素Fosfomycin	R
大观霉素 Spectinomycin	S	杆菌肽 Bacitracin	R	链霉素 Streptomycin	R	头孢丙烯 Cefprozil	R
哌拉西林/他唑巴坦 Piperacillin/tazobactam	S	罗美沙星 Lomefloxacin	R	新生霉素 Novobiocin	R	替加环素 Tigecycline	R
替卡西林/克拉维酸 Ticacillin/clavulanic acid	S	阿奇霉素 Azithromycin	R	阿洛西林 Azlocillin	R	克拉霉素 Clarithromycin	R
头孢他啶/克拉维酸 Ceftazidime/clavulanic acid	S	米诺环 Minocycline	R	痢特灵 Furazolidone	R	头孢美唑 Cefmetazole	R
头孢噻肟/克拉维酸 Cefotaxime/clavulanic acid	S	氧氟沙星 Ofloxacin	R	依诺沙星 Enoxacin	R	环丙沙星 Ciprofloxacin	R
头孢哌酮/舒巴坦 Cefoperazone/sulbactam	S	青霉素G Penicillin	R	头孢孟多 Cefamandole	R	头孢氨苄 Cephalexin	R
氨曲南Aztreonam	S

抗生素Antibiotics	HN2106	抗生素Antibiotics	HN2106	抗生素Antibiotics	HN2106	抗生素Antibiotics	HN2106
亚胺培南Imipenem	R	头孢西丁Cefoxitin	R	庆大霉素Gentamicin	R	奈替米星Netilmicin	R
氨苄西林Ampicillin	R	头孢克洛Cefaclor	R	卡那霉素Kanamycin	R	诺氟沙星Norfloxacin	R
海他西林Hetacillin	R	头孢呋辛Cefuroxime	R	阿米卡星Amikacin	R	新霉素Neomycin	R
阿莫西林 Amoxicillin	R	头孢噻肟 Cefotaxime	R	链霉素 Streptomycin	R	羧苄西林 Carbenicillin	R
苯唑西林Oxacillin	R	头孢唑肟ceftizoxime	R	多西环素Doxycycline	R	哌拉西林Piperacillin	R
氯唑西林 Cloxacillin	R	头孢曲松 Ceftriaxone	R	四环素 Tetracycline	R	妥布霉素 Tobramycin	R
美洛培南 Meropenem	R	头孢哌酮 Cefoperazone	R	氯霉素 Chloramphenicol	R	氟罗沙星 Fleroxacin	R
多黏菌素B Polymyxin B	R	头孢他啶 Ceftazidime	R	克林霉素 Clindamycin	R	头孢他美 Cefetamet	R
美洛西林/舒巴坦 Mezlocillin/Sulbactam	R	头孢噻呋 Ceftiofur	R	红霉素 Erythromycin	R	万古霉素 Vancomycin	R
阿莫西林/舒巴坦 Amoxicillin/sulbactam	R	头孢比肟 Cefepime	R	头孢羟氨苄 Cefadroxil	R	利福平 Rifampicin	R
阿莫西林/克拉维酸 Amoxicillin/clavulanic acid	R	头孢喹肟 Cefquinome	R	呋喃妥因 Nitrofurantoin	R	头孢地嗪 Cefodizime	R
磺胺甲异恶唑 Sulfamethoxazole	R	左氧氟沙星 Levofloxacin	R	头孢匹胺 Cefpiramide	R	头孢噻吩 Cephalothin	R
氨苄西林/舒巴坦 Ampicillin/Sulbactam	R	司帕沙星 Sparfloxacin	R	头孢克肟 Cefixime	R	依替米星 Etimicin	R
磺胺甲恶唑 Sulfamethoxazole	S	美洛西林 Mezlocillin	R	罗红霉素 Roxithromycin	R	替考拉宁 Teicoplanin	R
头孢美唑Cefmetazole	S	庆大霉素Gentamicin	R	加替沙星Gatifloxacin	R	磷霉素Fosfomycin	R
大观霉素 Spectinomycin	S	杆菌肽 Bacitracin	R	链霉素 Streptomycin	R	头孢丙烯 Cefprozil	R
哌拉西林/他唑巴坦 Piperacillin/tazobactam	S	罗美沙星 Lomefloxacin	R	新生霉素 Novobiocin	R	替加环素 Tigecycline	R
替卡西林/克拉维酸 Ticacillin/clavulanic acid	S	阿奇霉素 Azithromycin	R	阿洛西林 Azlocillin	R	克拉霉素 Clarithromycin	R
头孢他啶/克拉维酸 Ceftazidime/clavulanic acid	S	米诺环 Minocycline	R	痢特灵 Furazolidone	R	头孢美唑 Cefmetazole	R
头孢噻肟/克拉维酸 Cefotaxime/clavulanic acid	S	氧氟沙星 Ofloxacin	R	依诺沙星 Enoxacin	R	环丙沙星 Ciprofloxacin	R
头孢哌酮/舒巴坦 Cefoperazone/sulbactam	S	青霉素G Penicillin	R	头孢孟多 Cefamandole	R	头孢氨苄 Cephalexin	R
氨曲南Aztreonam	S