Effects of Hormonal and Adversely Stress on Vitamin E and γ-TMT Gene Expression in Soybeans

doi:10.13560/j.cnki.biotech.bull.1985.2023-0183

Abstract

Abstract:

The response of vitamin content and synthesis-related gene γ-TMT to hormonal and stress stress was clarified, which opened up new ideas for vitamin E to participate in the study of adversity stress. Taking Jinda 88, the main cultivars of Shanxi province, as the experimental materials, the vitamin E component content, physiological indexes and γ-TMT expression patterns in different tissues were analyzed under hormonal and adversity stress. The results showed that vitamin E was mainly present in the leaves, a small amount in stems, and absent in roots, and the total vitamin E content in leaves and stems was smaller than that in control under stress conditions such as hormones and salts, but the content of γ-tocopherols under NaCl, H₂O₂ and ABA stress was higher than that of the control. Under hormonal and adversity stress, the growth of soybean plants was significantly inhibited, and the plant height, fresh weight, dry weight, SPAD value and water content were all lower than those of the control. Genomic data were mined to 3 γ-TMTs, and there were many stresses response and hormone response elements in the promoters, and the expression of γ-TMT1-γ-TMT3 in roots and leaves was upregulated or down-regulated under hormonal and adversity stress. Under NaCl stress, the content of γ-tocopherol in soybean leaves increased, while the expression of γ-TMT2 and γ-TMT3 was significantly down-regulated, and it was speculated that γ-TMT2 and γ-TMT3 may affect the content of vitamin components under salt stress. Hormonal and adverse stress significantly inhibited the contents of vitamin E in soybean stems and leaves, which in turn inhibited plant growth, i.e.,affected soybean γ-TMT expression and vitamin E components.

Key words: soybean, vitamin E, γ-TMT, hormone, adversity

BAI Miao, TIAN Wen-qing, WU Shuai, WANG Min, WANG Li-xiang, YUE Ai-qin, NIU Jing-ping, ZHANG Yong-po, GAO Chun-yan, ZHANG Wu-xia, GUO Shu-jin, DU Wei-jun, ZHAO Jin-zhong. Effects of Hormonal and Adversely Stress on Vitamin E and γ-TMT Gene Expression in Soybeans[J]. Biotechnology Bulletin, 2023, 39(10): 148-162.

Figures/Tables 11

Table 1 Primers for RT-qPCR amplification of γ-TMT gene

基因名称 Gene name	引物 Primer	序列 Sequence（5'-3'）
γ-TMT1	F	GTGTGGTCCATGGAGAGTGG
γ-TMT1	R	CCCTGTGGCACCATGTTACT
γ-TMT2	F	CTGGCCGTCAGAGAATGGTT
γ-TMT2	R	GGAGCAACCAGTGTAGGCAT
γ-TMT3	F	TGACGCCCTAAAGCAACCAT
γ-TMT3	R	CCCTGTGGCACCATGTTACT
GmCYP2	F	CGGGACCAGTGTGCTTCTTCA
GmCYP2	R	GTGCTGCTACCCGAGCTAAT

Fig. 1 Amino acid sequence alignment of γ-TMT A: Alanine. R: Arginine. N: Asparagine. D: Aspartic acid. C: Cystine. Q: Glutarnine. E: Lutamic acid. G: Glycine. H: Histidine. I: Isoleucine. L: Leucine. K: Lysine. M: Methionine. F: Phenylalanine. P: Proline. S: Serine. T: Threonine. W: Tryptophan. Y: Tyrosine. V: Valine

Fig. 2 Prediction heat map of γ-TMT gene promoter cis-acting element

Fig. 3 SPAD values in hormonal and adversely stress environment Different lowercase letters indicate significant differences compared with the control（P<0.05）. The same below

Fig. 4 Determination of physiological indexes of plants under hormonal and adversely stress environment

Table 2 Regression equation and detection limit of tocopherol standard

标准品 Standard	回归方程 Regression equation	R²	检出限 Detection limit/（μg·g^-1）
α-生育酚 α-Tocopherol	Y=5.281 3X+0.104 2	1	0.17
γ-生育酚 γ-Tocopherol	Y=22.477X+0.258 3	0.999 7	0.03
δ-生育酚 δ-Tocopherol	Y=25.108X-1.6	0.999 8	0.03

Table 3 Analysis of vitamin E content in soybean leaves under hormonal and adversely stress environment

不同处理 Different treatment	α-生育酚 α-tocopherol/（μg·g^-1）	γ-生育酚 γ-tocopherol/（μg·g^-1）	δ-生育酚 δ-tocopherol/（μg·g^-1）	总维生素E Total vitamin E/（μg·g^-1）
CK	21.35±9.41a	93.04±42.07a	17.36±7.52a	131.75±55.16a
PEG	12.28±4.81b	89.35±34.07a	9.77±5.26b	111.40±43.08b
NaCl	15.26±0.29b	109.37±15.00b	10.32±0.44b	124.25±14.47a
H₂O₂	8.93±0.61b	105.83±7.39b	3.86±0.38b	118.62±8.26b
ABA	11.95±2.14b	106.33±7.45b	8.93±0.63b	127.21±8.77a
SA	11.96±3.21b	81.33±25.48b	9.22±1.11b	102.50±25.49b

Table 4 Analysis of vitamin E content in soybean stems under hormonal and adversely stress environment

不同处理 Different treatment	α-生育酚 α-tocopherol/（μg·g^-1）	γ-生育酚 γ-tocopherol/（μg·g^-1）	δ-生育酚 δ-tocopherol/（μg·g^-1）	总维生素E Total vitamin E/（μg·g^-1）
CK	0.00±0.00	8.63±0.69a	0.57±0.10a	9.20±0.64a
PEG	0.00±0.00	3.21±0.47b	0.68±0.22b	3.89±0.27b
NaCl	0.00±0.00	1.00±0.27b	0.69±0.11b	1.68±0.29b

Fig. 5 Analysis of γ-TMT1 gene expressions in hormonal and advsrsely stress environment A, D: Relative expression in the leaf and root under SA treated. B, E: Relative expression in the leaf and root under ABA treated. C, F: Relative expression in the leaf and root under H2O2 treated. G, I: Relative expression in the leaf and root under PEG treated. H, J: Relative expression in the leaf and root under NaCl treated. The same below

Fig. 6 Analysis of γ-TMT2 gene expressions in hormonal and adversely stress environment

Fig. 7 Analysis of γ-TMT3 gene expressions in hormonal and adversely stress environment

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