Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (2): 88-95.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0538

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OsDIS1 Negatively Regulates Rice Drought Tolerance Through Antioxidant Pathways

YANG Mao1,2(), LIN Yu-feng1,2, DAI Yang-shuo1,2, PAN Su-jun1,2, PENG Wei-ye1,2, YAN Ming-xiong1,2, LI Wei1,2, WANG Bing1,2(), DAI Liang-ying1,2()   

  1. 1. Hunan Agricultural University, Hunan Key Laboratory of Plant Disease and Insect Biology and Prevention and Control, Changsha 410128
    2. College of Plant Protection, Hunan Agricultural University, Changsha 410128
  • Received:2022-05-04 Online:2023-02-26 Published:2023-03-07

Abstract:

Drought, as an abiotic stress factor, seriously affects the yield and quality of rice. Exploring drought-related functional genes will help to breed new drought-resistance varieties. In this study, the Nipponbare OsDIS1-RNAi transgenic rice was constructed by Agrobacterium-mediated transformation method, and a series of physiological and biochemical experiments and agronomic traits analysis were conducted on it and wild type. The results showed that the activities of antioxidant enzymes after drought treatment significantly enhanced, the content of malondialdehyde significantly reduced and the expression of reactive oxygen species clearance gene significantly increased in OsDIS1-RNAi transgenic rice compared with the wild type. After ABA treatment, OsDIS1-RNAi transgenic plants did not show significantly morphological changes; these results indicated that OsDIS1-mediated drought tolerance may be independent of the ABA pathway. Meanwhile, agronomic traits such as plant height, spike length and yield of OsDIS1-RNAi rice plants did not change. The above results indicate that OsDIS1 negatively regulates drought tolerance in rice by activating the antioxidant pathways to increase the intracellular scavenging capacity of reactive oxygen species, and it may have potential applications for drought tolerance breeding in rice.

Key words: rice, OsDIS1, antioxidant enzyme, drought tolerance, breeding