Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (3): 196-205.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0714
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LIU Si-jia1,2(), WANG Hao-nan1,2, FU Yu-chen3, YAN Wen-xin1,2, HU Zeng-hui1,2, LENG Ping-sheng1,2()
Received:
2022-06-10
Online:
2023-03-26
Published:
2023-04-10
LIU Si-jia, WANG Hao-nan, FU Yu-chen, YAN Wen-xin, HU Zeng-hui, LENG Ping-sheng. Cloning and Functional Analysis of LiCMK Gene in Lilium ‘Siberia’[J]. Biotechnology Bulletin, 2023, 39(3): 196-205.
引物名称 Primer name | 引物序列 Primer sequence(5'-3') |
---|---|
LiCMK-F | ATGGCCTCCTCTCACCT |
LiCMK-R | TTATTCGGTTGCTGCTGCTG |
qLiCMK-F | CCGATAACTTCTTCTGGATTCATCT |
qLiCMK-R | GCCTGACCATTCTTGTAACTCTT |
qLiLIS-F | TCGCCTTGTTGAGATCATCCTCTTC |
qLiLIS-R | TGTCTCCACGGAGCTGCTGTT |
qLiOCS-F | TCCGCCAATTACCACCCAACCA |
qLiOCS-R | GCTGAGCCACTGGATCGTCTGA |
qLiMYS-F | GACTTGTTGGAGCCATGTCTTAGAGA |
qLiMYS-R | AACTCAACTCGTCGCCAAGAAGT |
β-Actin-F | CGGTGTCTGGATTGGAGGGTCA |
β-Actin-R | TTCGCTTTAGGACTTCGGGT |
LiCMK-SF | AGTGGTCTCTGTCCAGTCCTATGGCCTCCTCTCACCT |
LiCMK-SR | GGTCTCAGCAGACCACAAGTTTCGGTTGCTGCTGCTG |
LiCMK-TF | AGTGGTCTCTGTCCAGTCCTATGGCCTCCTCTCACCT |
LiCMK-TR | GGTCTCAGCAGACCACAAGTCAACCCAGCCTGCTTATCC |
Table1 Primer sequences
引物名称 Primer name | 引物序列 Primer sequence(5'-3') |
---|---|
LiCMK-F | ATGGCCTCCTCTCACCT |
LiCMK-R | TTATTCGGTTGCTGCTGCTG |
qLiCMK-F | CCGATAACTTCTTCTGGATTCATCT |
qLiCMK-R | GCCTGACCATTCTTGTAACTCTT |
qLiLIS-F | TCGCCTTGTTGAGATCATCCTCTTC |
qLiLIS-R | TGTCTCCACGGAGCTGCTGTT |
qLiOCS-F | TCCGCCAATTACCACCCAACCA |
qLiOCS-R | GCTGAGCCACTGGATCGTCTGA |
qLiMYS-F | GACTTGTTGGAGCCATGTCTTAGAGA |
qLiMYS-R | AACTCAACTCGTCGCCAAGAAGT |
β-Actin-F | CGGTGTCTGGATTGGAGGGTCA |
β-Actin-R | TTCGCTTTAGGACTTCGGGT |
LiCMK-SF | AGTGGTCTCTGTCCAGTCCTATGGCCTCCTCTCACCT |
LiCMK-SR | GGTCTCAGCAGACCACAAGTTTCGGTTGCTGCTGCTG |
LiCMK-TF | AGTGGTCTCTGTCCAGTCCTATGGCCTCCTCTCACCT |
LiCMK-TR | GGTCTCAGCAGACCACAAGTCAACCCAGCCTGCTTATCC |
Fig. 7 Prediction of secondary structure of LiCMK protein in Lilium ‘Siberia’ The blue is α-helix, red is extended strand, green is beta turn, and purple is random coil
Fig. 9 Expression analysis of LiCMK in Lilium ‘Siberia’ at different flowering stages and LiCMK in different tissues The error line in the figure refers to the standard deviation. Different lowercase letters indicate significantly different at P<0.05. The same below
Fig. 10 Subcellular localization of LiCMK protein in Lilium ‘Siberia’ There are the target protein fluorescence channel, bright field, chloroplast fluorescence channel and merged one from left to right
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