Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (1): 168-175.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0691
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YANG Hong-yan(), HAN Xiao, YANG Jian-jun()
Received:
2023-07-17
Online:
2024-01-26
Published:
2024-02-06
Contact:
YANG Jian-jun
E-mail:jessy.yang@cytiva.com;jianjun.yang@cytiva.com
YANG Hong-yan, HAN Xiao, YANG Jian-jun. Scaling up Production of pDNA Plasmids in Disposable Bioreactors[J]. Biotechnology Bulletin, 2024, 40(1): 168-175.
基础培养基 Medium type | 最高菌体密度 Peak OD600 | 质粒产量 Plasmid titer/(mg·L-1) | 单位菌体密度质粒产量 Specific plasmid yield /(mg·L-1·OD600-1) |
---|---|---|---|
LB培养基 LB basic medium | 6.07 | 11.29 | 1.86 |
Opti培养基 Opti basic medium | 8.66 | 19.98 | 2.31 |
Table 1 Performance comparison between LB Basic Medium and Opti Basic Medium
基础培养基 Medium type | 最高菌体密度 Peak OD600 | 质粒产量 Plasmid titer/(mg·L-1) | 单位菌体密度质粒产量 Specific plasmid yield /(mg·L-1·OD600-1) |
---|---|---|---|
LB培养基 LB basic medium | 6.07 | 11.29 | 1.86 |
Opti培养基 Opti basic medium | 8.66 | 19.98 | 2.31 |
培养装置 Seed expansion vessel | 培养时间 Culture time/h | 菌体密度OD600 | 平均比生长速率Average specific growth rate /(h-1) | 关键工艺参数是否可控和可记录 Parameters controllable and recordable |
---|---|---|---|---|
三角摇瓶Shake flask | 10.2 | 1.01 | 0.72 | No |
WAVE25生物反应器WAVE25 bioreactor | 11.0 | 1.20 | 0.66 | Yes |
Table 2 Comparison of E. coli seed expansion between shake flask and WAVE25 bioreactor
培养装置 Seed expansion vessel | 培养时间 Culture time/h | 菌体密度OD600 | 平均比生长速率Average specific growth rate /(h-1) | 关键工艺参数是否可控和可记录 Parameters controllable and recordable |
---|---|---|---|---|
三角摇瓶Shake flask | 10.2 | 1.01 | 0.72 | No |
WAVE25生物反应器WAVE25 bioreactor | 11.0 | 1.20 | 0.66 | Yes |
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