Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (1): 176-185.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0699

Previous Articles     Next Articles

Advances in the Biological Modification of Galactose Oxidase

XIA Guang-li1,2(), CAO Na1,2, SUN Hui-hui2(), ZHAO Ling2, CAO Rong2,3   

  1. 1. College of Food Science and Engineering, Ocean University of China, Qingdao 266404
    2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071
    3. Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237
  • Received:2023-07-19 Online:2024-01-26 Published:2024-02-06
  • Contact: SUN Hui-hui E-mail:18765600710@139.com;sunhh@ysfri.ac.cn

Abstract:

As an important biological catalyst, galactose oxidase has the characteristics of high catalytic efficiency and environmental protection. It has been widely used in biosensors, food, medical treatment, chemical industry and other fields. However, natural galactose oxidase has some inherent defects such as poor stability, low catalytic efficiency, and high recovery cost, which greatly limit its application in food safety, biomedicine, and other fields. With the development of genetic engineering and protein engineering, galactose oxidase can be purposefully modified to improve the performance of the enzyme or to obtain recombinant enzymes with new functions. Therefore, the pursuit of galactose oxidase with important catalytic capacity and powerful functions has become a research hotspot in this field. In this paper, the molecular structure and catalytic mechanism of galactose oxidase are reviewed, and the relevant cases of biological modification of galactose oxidase are analyzed and summarized, aiming to provide new ideas and methods for galactose oxidase related researchers.

Key words: galactose oxidase, biological modification, rational design, directed evolution, immobilization