Research on the Factors Related to Agrobacterium-mediated Genetic Transformation of the Chinese Chive

Abstract

Abstract: Using “Hanzhong Dongjiu” variety and the Agrobacterium EHA105 containing pCAMBIA3301 plasmid as the material, it studied the factors which could influence transformation efficiency of Chinese chive. The results showed that transient expression rate of GUS gene reached 93% with the callus cultured for 40 d as the explant and the adventitious buds differentiated well. When 100 μmol/L acetosyringone was added to the miedium, GUS expression rate of the callus reached 91% and the plant regeneration rate was 7.9%, but did not increase with increasing of the AS concentration. Compared with other combinations, the calli were soaked in Agrobacterium(OD600 ≈ 0.6)for 10 min, the injury degree of explants was slight and the GUS expression rate and regeneration rate were the highest. When the calli were co-cultured for 3 days after infection, the bacterium grew less than other period, GUS expression rate and the regeneration rate were 91.1% and 7.2% respectively. Through the experiment, the best conditions of genetic transformation factors for chinese chive were obtained.

Key words: Chinese chive, Agrobacterium, GUS staining, Genetic transformation

Chang Jing, Gao Xingying, Li Xiaodong, Hou Leiping, Li Meilan. Research on the Factors Related to Agrobacterium-mediated Genetic Transformation of the Chinese Chive[J]. Biotechnology Bulletin, 2014, 0(1): 125-131.

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