Expression Profiles of Pathogen-related Protein Gene(SfPR-1)from Salsola ferganica and Construction of Plant#br#Expression Vectors

Abstract

Abstract: To investigate the potential roles of SfPR-1 at different developmental stages, tissues, phytohormones treatment, different abiotic stresses and biotic stress treatment, semi-quantitative RT-PCR method was employed to explore the expression profiles of pathogenesisrelated protein gene SfPR-1(GenBank accession number: JQ670917)from halophytes Salsola ferganica. The results displayed that SfPR-1 gene was mainly expressed in root tissue with low expression in the stem and leaves, suggesting that the gene may play a major role in the root defense. The expression characteristic of SfPR-1 at different developmental stages(seed, 20 d shoots after seed germination, 30 d shoots after seed germination, 40 d shoots after seed germination)showed the highest level was in 30 d shoots after seed germination, indicating that may play an important role in the plant late growth. The expression of SfPR-1 generated with the application of phytohormones including salicylic acid(SA), jasmonate(JA), 1-aminocyclopropane-1-carboxylate(ACC), and abscisic acid(ABA). This response can also be induced by abiotic stresses including H2O2, salt, drought, cold, and biotic stresses such as Penicillium italicum infection. The transcript levels of SfPR-1 substantially increased during biotic stress during 48 h treatment. These results suggested that SfPR-1was a key gene to the biotic and abiotic response and may play an important role in host plant defense and environmental stress.

Key words: Salsola ferganica, SfPR-1 gene, RT-PCR, Expression profiles, Construction of plant expression vectors

Wang Yan, Chen Xi, Zhou Lianjie, Yang Zhongmin. Expression Profiles of Pathogen-related Protein Gene(SfPR-1)from Salsola ferganica and Construction of Plant#br#Expression Vectors[J]. Biotechnology Bulletin, 2014, 0(1): 116-124.

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