Involvement of Antioxidant Defense System in Drought and Chilling Comprehensive Resistance Formation in Different#br#Resistant Varieties of Tobacco Seedlings

Abstract

Abstract: Two varieties of tobacco with different drought/chilling-resistance, MSK326 and yunyan203, were taken as experimental materials in this study, aiming at understanding the involvement of antioxidant defense system in drought and chilling comprehensive resistance formation of tobacco seedlings. MSK326 had a higher viabillity than yunyan203, with a lower relative electrolytic leakage and MDA content of leaves under both drought and chilling stress, demonstrating a higher comprehensive resistance to drought and chilling stress than yunyan203. The results of cellular antioxidant contents and enzymatic activity determination showed that, under drought or chilling stress, MSK326 could retain higher contents of AsA and GSH, ratio of reduced to total antioxidants and activities of the antioxidant enzymes(SOD, CAT, POD,APX)than yunyan203, especially at the late stage of stress. All these results indicated that an enhanced intracellular anti-oxidative capacity of MSK326 could be helpful to alleviate stress-induced oxidative damages, indicating the involvement of antioxidant system in drought and chilling comprehensive resistance formation of tobacco seedlings.

Key words: Antioxidant defense system, Tobacco, Drought stress, Chilling stress, Comprehensive resistance

Wang Shasha, Sheng Yelong, Ma Wenguang, Hao Dahai, Zhang Jianbo, Gong Ming. Involvement of Antioxidant Defense System in Drought and Chilling Comprehensive Resistance Formation in Different#br#Resistant Varieties of Tobacco Seedlings[J]. Biotechnology Bulletin, 2014, 0(1): 132-142.

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