Phylogenetic Analysis of Three Domestic Pig Breeds in Guizhou Province

Abstract

Abstract: PCR product of the complete mitochondrial control regions of 57 pigs from 3 Chinese pig breeds in Guizhou(Baixi pig, Congjiangxiang pig and Qianbeihei pig)were directly sequenced, the genetic distance between the mtDNA of three breeds of pig with other 25 pig breeds was calculated, and the phylogenetic tree was built. The results revealed that, the sequences of the mtDNA D-loop of the three pig breeds were 1 254-1 314 bp, the genetic distance between the three breeds was 0.002 9-0.007 2, comparing Baixi pig with Qianbei Black pig and Congjiang xiang pig, the genetic distance of Baixi pig was great, all of 0.007 2. The results of phylogenetic anslysis supported the view that the Chinese pig breeds respectively originated from the Southern and the Northern, and there were gentic exchanging among the Chinses pig breeds.

Key words: mt, DNA, D-loop, Genetic distance, Domestic pig breeds, Phylogeny

Ding Mei, Chen Xiang, Xu Houqiang, Wu Tonggui, Feng Wenwu, Bai Lin,. Phylogenetic Analysis of Three Domestic Pig Breeds in Guizhou Province[J]. Biotechnology Bulletin, 2014, 0(4): 96-101.

References

[1] MacKay SLD, Oliver PD, Laipis PJ. Template directed arrest of mammalian mitochondrial DNA synthesis[J]. Molecular Cell Biology, 1986, 6：1261-1267.
[2] Grossi SF, Lui JF, Garcia JE, et al. Genetic diversity in wild(Sus scrofa scrofa)and domestic(Sus scrofa domestica)pigs and their hybrids based on polymorphism of a fragment of the D-loop region in the mitochondrial DNA[J]. Genet Mol Res, 2006, 5(4)：564-568.
[3] Royo LJ, Alvarez I, Beja-Pereira A, et al. The origins of Iberian horses assessed via mitochondrial DNA[J]. Journal of Heredity, 2005, 96(6)：663-669.
[4] 孙俊丽, 张冰, 马青艳, 等. 陆川猪mtDNA D-loop 序列遗传多样性分析[J].中国畜牧兽医, 2010, 37(6)：122-125.
[5] 苟潇, 亐开兴, 吴桂生, 等. 云南4个地方猪品种mtDNA多态及其起源分化研究［C]//中国畜牧兽医学会2004年学术年会暨第五届全国畜牧兽医青年科技工作者学术研讨会论文集(上册). 北京：中国畜牧兽医学会, 2004：237-241.
[6] 冯文豪. 贵州三个地方猪种遗传多样性及亲缘关系研究［D]. 贵州：贵州大学, 2008.
[7] Yang J, Wang J, Kijas J, et al. Genetic diversity present within the near-completemt DNA genome of 17 breeds of indigenous Chinese pigs[J]. Journal of Heredity, 2003, 94(5)：381-385.
[8] 王继英, 郭建凤, 孙守礼, 等. 山东猪种mtDNA D-loop区部分序列遗传多样性及系统进化研究[J].畜牧兽医学报, 2009, 40(6)：792-799.
[9] 刘娣, 杨秀芹, 杨甲芳, 等. 野猪及其与家猪杂种猪Myostatin基因3'编码区的克隆和序列分析[J]. 兽类学报, 2003, 23(3)：270-272.
[10] Watanabe T, Havashi Y, Ogasawara N, et al. Polymorphism of mitochondrial DNA in pigs based on restriction endonuclease cleavage patterns[J]. Biochem Genet, 1985, 23：105-1 13.
[11] Giuffra E, Kijias JMH, Amarger V, et al. The origin of the domestic pig：independent domestication and subsequent introgression[J]. Genetics, 2000, 154(4)：1785-1791.
[12] Kijias JMH, Andersson L. A phylogenetic study of the origin of the domestic pig estimated from the near-complete mtDNA genome[J]. J Mol Evol, 2001, 52(3)：302-308.
[13] 张冬杰, 刘娣, 杨国伟, 等.野猪骨桥蛋白基因表达及与家猪的对比分析[J]. 动物学杂志, 2009, 44(2)：41-47.
[14] 黄勇富, 张亚平, 邱祥聘, 等. 猪线粒体DNA多态性与中国地方猪种起源分化的关系[J]. 遗传学报, 1998, 25(4)：322-329.
[15] 苟潇, 连林生. 动物数量育种中的分子标记辅助选择[J]. 动物科学与动物医学, 2001, 19(5)：14-16.
[16] 李洪涛, 肖东, 袁进, 等. 西藏小型猪线粒体DNA控制区的分子遗传学研究[J]. 畜牧兽医学报, 2008, 40(6)：800-805.
[17] 刘晓辉. 利用Y染色体研究中国家猪的起源与驯化［D]. 北京：中国农业大学, 2005.