Analyzing Polymorphism of SLA-DQA Gene Exon 2 in the Hezuopig

Abstract

Abstract: The polymorphism, allele number, nucleotide polymorphism sites, amino acid polymorphism sites, the genetic relationship and evolutionary significance of the alleles of the SLA-DQA gene exon 2 in Hezuo pigs were studied. Variation of SLA-DQA gene in the 439 Hezuopigwas investigated by amplification of exon 2 using PCR, followed by single-strand conformational polymorphism(SSCP), DNA sequencing and sequence data analysis. Result showed that seven alleles were found in the SLA-DQA geneexon 2. Eighteen nucleotide polymorphism sites and ten amino acid polymorphism sites were identified in seven SLA-DQA gene exon 2 haplotypes. The study indicated that a high level of sequence polymorphism existed in SLA-DQA gene exon 2 of Hezuo pigs, and the population might have more genetic resources. SLA-DQA gene exon 2 in Hezuo pigs might be differentiated into two major categories alleles from one mutant alleles at first. SLA-DQA gene exon 2 in Hezuo pigs and other pig had high homology, which indicated that SLA-DQA gene exon 2 in pig might come from primordial sequences that were present in a common ancestor and persisted in the pig populations since their divergence. Seven new alleles that had near genetic relationship, may came from the two near allele by mutation.

Key words: Hezuopig, SLA-DQA gene exon 2, Polymorphism, PCR-SSCP

Zhang Guohua, Ma Xiaojun, Lü Weili,. Analyzing Polymorphism of SLA-DQA Gene Exon 2 in the Hezuopig[J]. Biotechnology Bulletin, 2014, 0(4): 91-95.

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