Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (4): 147-151.

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Isolation and Characterization of Endophytic Strains Producing Exopolysaccharide

Huang Yicheng, Liu Yang, Pang Xin, Ma Zhongrui, Han Donglei, Chen Min   

  1. (The State Key Laboratory of Microbial Technology,School of Life Sciences,Shandong University,Jinan 250100)
  • Received:2013-10-09 Online:2014-04-29 Published:2014-04-29

Abstract: In this study, 24 strains were isolated from carrot, aloe, potato, sweet potato and purple potato. As screening by phenol-sulfuric acid assay, three strains, 25-Z-1, 25-Z-3 and 12-L-6, were proved to be as high exopolysaccharide producing strains. The results of 16S rDNA sequence determination showed that 25-Z-1 and 25-Z-3 isolated from purple potato was about 100% identity compared with Bacillus pumilus strain ST277 and 99% identity compared with Bacillus cereus strain Se05, respectively. Meanwhile the 12-L-6 isolated from aloe identified as Bacillus sp. GZT, with the similarity is of 99%. Culture in LB medium, 30℃ and 170 r/min for 7 d, yield of EPS could reach to 30.3 mg/L, 29.1 mg/L and 28.2 mg/L, respectively.

Key words: Endophyte, Exopolysaccharide, 16S, rDNA