Research Progress of Improving the Actinomycetes Secondary Metabolite Production Method

Abstract

Abstract: Actinomycetes secondary metabolites have been the important sources of developing antibiotics and other drugs. Gene technology has been widely practiced for improving the yield of Actinomycetes secondary metabolites. This review summarized a series of technical methods used for improving Actinomycetes secondary metabolites，consisting of over-expressing the key structural genes and/or positive regulation genes，knocking out the negative regulatory genes，doubling the clusters，mutating ribosomal protein genes，and expressing the foreign cluster heterologously. These methods could be used for new drug discovery and strains improvement.

Key words: Secondary, Netabolites, Ribosome engineering, Heterologous expression, Actinomycetes

Lei Xiuqing, Li Li, Huang Jianzhong. Research Progress of Improving the Actinomycetes Secondary Metabolite Production Method[J]. Biotechnology Bulletin, 2014, 0(5): 45-51.

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