Optimization of β-cyclodextrin Production by Recombinant β-cyclodextrin Glycosyltransferase

Abstract

Abstract:

The βcgt gene encoding β-CGTase from Bacillus circulans strain 251 was cloned into the expression vector pET-20b（+）.The vector was then transformed into Escherichia coli BL21（DE3）for extracellular production of β-CGTase. The activity in the supernatant of recombinant E. coli BL21（DE3）was 20 U/mL. Furthermore, the condition for β-cyclodextrin（β-CD）preparation by this recombinant β-CGTase was optimized. At 15% potato starch, pH5.5, 30℃, 2.5%-5%（V/V）cyclohexane, 10 U β-CGTase per gram substrate incubated for 24 hours, 75.3% of the starch was transformed into β-CD. This is the highest level of β-CD conversion by enzyme method at home and abroad.

Key words: Cyclodextrin glycosyltransferase, β-cyclodextrin, Enzymatic conversion, Recombinant expression, Starch

Yang Yulu, Wang Lei, Chen Sheng, Wu Jing. Optimization of β-cyclodextrin Production by Recombinant β-cyclodextrin Glycosyltransferase[J]. Biotechnology Bulletin, 2014, 0(8): 175-181.

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