EMS Mutagenesis，Mutant Screening and Identification of Sorghum

Abstract

Abstract: Mutation rate and mutation type are important bases for phenotype screening and constructing saturation mutant library. Different treating time and different concentrations of Ethylmethylsulfone（EMS）for BTx623 seeds have been used in this study, mutation rate and mutation type are quite different. Mutation types are very rich, including leaf color, leaf shape, spike type, fertility, and growth period. According to the emergence rate, seed rate and mutation rate, 20 h+EMS 0.2% is considered the optimum treating time and concentration. However, if we want to build a saturated mutant library, different treating-time and various concentrations should be used.

Key words: Sorghum bicolor, Ethylmethylsulfone（EMS） , Mutant Mutation rate , Mutation type

Wang Chunyu,Zhu Zhenxing, Li Dan, Cong ling,Zhang Lixia. EMS Mutagenesis，Mutant Screening and Identification of Sorghum[J]. Biotechnology Bulletin, 2014, 0(9): 78-83.

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