Prokaryotic Expreesion and RNA Interference Vector Construction for Geranyl Diphosphate Synthase of Mentha haplocalyx Briq.

Abstract

Abstract: Geranyl diphosphate synthase（GPPS）gene total ORF（1 131 bp）was amplified with specific primers and linked to expression vector pET-28a. Verified by double restriction enzyme digestion and sequencing, recombined vector pET-28a-GPPS was transferred to Rosetta（DE3）and the recombined protein was overexpressed after 6 h induced by 1 mmol/L IPTG. Using RNA interference method to research gene function is matured gradually. In our research, the expression vector pBI121-RNAi-GPPS targeting to GPPS gene was structured successfully.

Key words: Mentha haplocalyx Briq, GPPS , Prokaryotic expreesion , RNAi , Vector construction

Yu Xu,Liang Chengyuan, Liu Yan, Li Weilin. Prokaryotic Expreesion and RNA Interference Vector Construction for Geranyl Diphosphate Synthase of Mentha haplocalyx Briq.[J]. Biotechnology Bulletin, 2014, 0(9): 84-88.

References

［1］Dudareva N, Negre F, Nagegowda DA, Orlova I. Plant volatiles：recent advances and future perspectives［J］. Crit Rev Plant Sci, 2006（25）：417-440.
［2］Serna L, Marin C. Trichomes：different regulatory networks lead to convergent structures［J］. Trends Plant Sci, 2006, 11：274-280.
［3］中华人民共和国卫生部国家药典委员会.中华人民共和国药典：（2010年版一部）［M］.北京：化学工业出版社人民卫生出版社, 2010.
［4］彭蕴茹, 钱士辉, 石磊, 等. 薄荷非挥发性提取部位的药理活性研究［J］. 中药材, 2008, 31（1）：104-107.
［5］王晖, 许卫铭, 王宗锐.薄荷醇对柴胡镇痛成分表观生物利用度的影响［J］.中成药, 1996, 18（6）：4-6.
［6］Lee MY, Lee JA, Seo CS, et al. Protective effect of Mentha haplocalyx ethanol extract（MH）in a mouse model of allergic asthma［J］. Phytotherapy Research, 2011, 25：863-869.
［7］Chan BC, Hong KL, Leung PC, et al. Traditional Chinese medicine for a topic eczema：PentaHerbs formula suppresses inflammatory mediators release from mast cells［J］. Journal of Ethnopharmacol-ogy, 2008, 120：85-91.
［8］Croteau R. Biosynthesis and catabolism of monoterpenoids［J］. Chem Rev, 1987, 87：929-954.
［9］Burke C, Wildung MR, Croteau R. Geranyl diphosphate synthase：cloning expression and characterization of this prenyltransferase as a heterodimer［J］.Proc Natl Acad Sci USA, 1999（96）：13062-13067.
［10］Burke C, Klettke K, Croteau R. Heteromeric geranyl diphosphate synthase from mint：construction of a functional fusion protein and inhibition by bisphosphonate substrate analogs［J］. Proc Natl Acad Sci USA, 2004, 422：52-60.
［11］Gong D, Zhang C, Chen X, et al. Constitutive activation and transgenic evaluation of the function of an Arabidopsis PKS protein kinase［J］. J Biol Chem, 2002, 277（44）：42088-42096.
［12］Xu M, Zhu L, Shou H, et al. A PIN1 family gene, OsPIN1, involved in auxin-dependent adventitious root emergence and tillering in rice［J］. Plant & Cell Physiology, 2005, 4（610）：1674-1681.
［13］Mahmoud SS, Croteau RB. Menthofuran regulates essential oil biosynthesis in peppermint by controlling a downstream monoterpene reductase［J］. PNAS, 2003, 100（24）：14481-14486.
［14］Rodney BC, Edward MD, Kerry L, et al. Menthol biosynthesis and molecular genetic［J］. Naturwissenschaften, 2005, 92：562-577.
［15］Burke C, Croteau R. Geranyl diphosphate synthase from Abies grandis：cDNA isolation, functional expression, and characteriza-tion［J］. Archives of Biochemistry and Biophysics, 2002, 405：130-136.
［16］Bass BL. Double-stranded RNA as a template for gene silencing［J］. Cell, 2000, 101：235-238.
［17］王永佳, 陈永胜, 李国瑞. RNAi在植物中的作用机理及其应用研究进展［J］.内蒙古民族大学学报：自然科学版, 2010, 25（1）：54-57.

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