Effects of EMS Mutagenesis on the Seeding Survival and Major Agronomic Traits of Sorghum in M1 Generation

doi:10.13560/j.cnki.biotech.bull.1985.2022-1499

Abstract

Abstract:

The current study mainly explored the effects of different EMS concentration on seedling survival and major agronomic traits in sorghum M₁ generation, and found the optimal EMS concentration in different sorghum varieties. It can provide the basic varieties and theoretical guidance on the mutant construction, the germplasm resources innovation and the new varieties cultivation with high yield and quality in sorghum. Six sorghum restorer lines were used in this study. The seeds were firstly soaked by EMS with different concentrations（0, 0.20%, 0.25%, 0.30%, and 0.35%）for 12 h. The seedling survival and related agronomic traits were investigated after sowing in the field. With the EMS concentration increasing, the seedling survival rate decreased gradually, while the mutation rate of plant height, leaf, panicle type, fertility and maturity stage increased in mutagenesis plants. In sorghum, 0.25% EMS was the optimal concentration of 1121R, LgB7B Daligao, L zao 615·L17, and 28R·Ji16. The 0.35% EMS was the optimal concentration of L17, while the optimal concentration of Digan L17 was higher than that under 0.35% EMS. Thus, EMS treatment has a great effect on seedling survival and major agronomic traits in sorghum, and it has different effects on different varieties.

Key words: sorghum（Sorghum bicolor L.）, EMS, mutation rate, agronomic trait

FAN Xin-qi, WANG Hai-yan, CHEN Jing, ZHANG Xiao-juan, GUO Qi, LIANG Du, ZHOU Fu-ping, NIE Meng-en, ZHANG Yi-zhong, LIU Qing-shan. Effects of EMS Mutagenesis on the Seeding Survival and Major Agronomic Traits of Sorghum in M₁ Generation[J]. Biotechnology Bulletin, 2023, 39(7): 173-184.

Figures/Tables 12

References 33

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EMS处理浓度 EMS treatment concentration/%	相对成苗率 Relative seeding emergence rate/%
EMS处理浓度 EMS treatment concentration/%	1121R	LgB7B大粒高 LgB7B Daligao	L早615·L17 L Zao 615·L17	低秆L17 Digan L17	L17	28R·吉16 28R·Ji16
0.00	100.0±0.00Aa	100.00±0.00Aa	100.00±0.00Aa	100.00±0.00Aa	100.00±0.00Aa	100.00±0.00Aa
0.20	75.64±0.65Ba	52.50±0.85Bc	75.43±0.73Ba	68.94±0.80Bb	71.81±1.07Bb	54.86±0.16Bc
0.25	74.36±0.41Ba	51.39±0.24BCd	65.43±0.54Cc	68.3±0.50Bb	63.61±0.55Cc	50.00±0.36Cd
0.30	59.23±0.81Cb	49.44±1.04Cd	54.78±0.72Dc	64.68±1.72BCa	58.07±0.67Db	45.71±0.36De
0.35	47.44±0.76Dd	43.06±0.51De	50.00±0.41Ec	61.23±0.61Ca	55.18±0.22Eb	42.86±0.52Ee

EMS处理浓度 EMS treatment concentration/%	相对成苗率 Relative seeding emergence rate/%
EMS处理浓度 EMS treatment concentration/%	1121R	LgB7B大粒高 LgB7B Daligao	L早615·L17 L Zao 615·L17	低秆L17 Digan L17	L17	28R·吉16 28R·Ji16
0.00	100.0±0.00Aa	100.00±0.00Aa	100.00±0.00Aa	100.00±0.00Aa	100.00±0.00Aa	100.00±0.00Aa
0.20	75.64±0.65Ba	52.50±0.85Bc	75.43±0.73Ba	68.94±0.80Bb	71.81±1.07Bb	54.86±0.16Bc
0.25	74.36±0.41Ba	51.39±0.24BCd	65.43±0.54Cc	68.3±0.50Bb	63.61±0.55Cc	50.00±0.36Cd
0.30	59.23±0.81Cb	49.44±1.04Cd	54.78±0.72Dc	64.68±1.72BCa	58.07±0.67Db	45.71±0.36De
0.35	47.44±0.76Dd	43.06±0.51De	50.00±0.41Ec	61.23±0.61Ca	55.18±0.22Eb	42.86±0.52Ee

EMS处理浓度 Treatments concentration/%	影响程度 Effect degree
0.00	1121R=L早615·L17>L17=低秆L17>LgB7B大粒高=28R·吉16
0.20	1121R>低秆L17>L早615·L17= L17>LgB7B大粒高=28R·吉16
0.25	低秆L17>1121R=L17>L早615·L17> LgB7B大粒高>28R·吉16
0.30	低秆L17>L17>L早615·L17> 1121R>LgB7B大粒高=28R·吉16

EMS处理浓度 Treatments concentration/%	影响程度 Effect degree
0.00	1121R=L早615·L17>L17=低秆L17>LgB7B大粒高=28R·吉16
0.20	1121R>低秆L17>L早615·L17= L17>LgB7B大粒高=28R·吉16
0.25	低秆L17>1121R=L17>L早615·L17> LgB7B大粒高>28R·吉16
0.30	低秆L17>L17>L早615·L17> 1121R>LgB7B大粒高=28R·吉16

EMS处理浓度 EMS treatment concentration/%	株高变异率 Plant height mutation rate/%
EMS处理浓度 EMS treatment concentration/%	1121R	LgB7B大粒高 LgB7B Daligao	L早615·L17 L Zao 615·L17	低秆L17 Digan L17	L17	28R·吉16 28R·Ji16
0.00	100.0±0.00Aa	100.0±0.00Aaa	100.0±0.00Aa	100.0±0.00Aa	100.0±0.00Aa	100.0±0.00Aa
0.20	17.59±0.79Bab	21.29±0.28Ca	7.83±0.20Cb	23.68±0.32Ca	21.64±4.86Ba	11.25±0.49Bb
0.25	33.12±0.49Cb	34.13±0.85Db	23.92±0.13De	27.16±0.21Dd	39.21±0.22Ca	30.86±0.54Cc
0.30	57.57±0.64Da	39.61±0.36Ecd	30.42±0.63Ee	36.31±0.37Ed	41.92±0.68Cc	48.00±1.45Db

Effects of EMS Mutagenesis on the Seeding Survival and Major Agronomic Traits of Sorghum in M₁ Generation

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材料 Materials	EMS处理浓度 EMS treatment concentration/%	育性变异率Fertility mutation rate/%
材料 Materials	EMS处理浓度 EMS treatment concentration/%	育性=0% Fertility=0%	育性≤10% Fertility≤10%	10%<育性≤50% 10%<Fertility≤50%	育性>50% Fertility>50%
1121R	0.00	0.00±0.00	0.00±0.00	0.00±0.00	100.00±0.00
	0.20	13.79±0.39	25.91±0.44	80.40±0.97	89.27±1.64
	0.25	14.85±0.37	25.58±0.39	11.60±0.29	5.75±0.17
	0.30	12.47±0.37	21.59±0.65	4.40±0.10	3.07±0.12
	0.35	58.89±0.33	26.91±0.42	3.60±0.08	1.92±0.22
LgB7B大粒高 LgB7B Daligao	0.00	0.00±0.00	0.00±0.00	0.00±0.00	100.00±0.00
	0.20	28.74±0.34	45.00±0.76	49.59±0.72	66.67±1.28
	0.25	32.93±0.42	7.14±0.26	4.88±0.21	4.44±0.12
	0.30	25.75±0.46	30.71±0.41	38.21±0.54	24.44±1.19
	0.35	12.57±0.42	17.14±0.94	7.32±0.20	4.44±0.18
L早615·L17 L Zao 615·L17	0.00	0.00±0.00	0.00±0.00	0.00±0.00	100.00±0.00
	0.20	16.88±0.53	41.94±0.7	68.94±0.47	84.11±1.14
	0.25	8.75±0.09	28.39±0.57	9.09±0.16	4.67±0.20
	0.30	5.63±0.55	9.68±0.25	6.82±0.33	5.61±0.21
	0.35	68.75±0.39	20.00±0.59	15.15±0.43	5.61±0.45
低秆L17 Digan L17	0.00	0.00±0.00	0.00±0.00	0.00±0.00	100.00±0.00
	0.20	9.01±0.21	13.47±0.28	17.22±0.37	17.24±0.56
	0.25	20.12±0.22	27.35±0.24	14.90±0.45	23.79±0.81
	0.30	22.82±0.33	33.47±0.22	19.87±0.49	28.28±0.42
	0.35	48.05±0.42	25.71±0.34	48.01±0.41	30.69±0.79
L17	0.00	0.00±0.00	0.00±0.00	0.00±0.00	100.00±0.00
	0.20	11.70±0.32	17.80±0.72	35.58±0.52	43.65±0.99
	0.25	9.43±0.34	15.53±0.80	32.69±0.73	26.52±0.95
	0.30	16.23±0.32	21.59±0.93	17.31±0.45	15.47±0.72
	0.35	62.64±0.51	45.08±0.86	14.42±0.57	14.36±0.88
28R·吉16 28R·Ji16	0.00	0.00±0.00	0.00±0.00	0.00±0.00	100.00±0.00
	0.20	34.93±1.25	46.63±0.65	50.36±0.65	93.55±1.51
	0.25	32.88±0.40	23.03±0.47	22.30±0.54	6.45±0.14
	0.30	23.29±1.09	12.92±0.51	17.27±0.52	0.00±0.00
	0.35	8.90±0.18	17.42±0.61	10.07±0.16	0.00±0.00

EMS处理浓度 EMS treatments concentration/%	叶片变异率 Leaf mutation rate/%
EMS处理浓度 EMS treatments concentration/%	1121R	LgB7B大粒高 LgB7B Daligao	L早615·L17 L Zao 615·L17	低秆L17 Digan L17	L17	28R·吉16 28R·Ji16
0.00	0.00±0.00Aa	0.00±0.00Aa	0.00±0.00Aa	0.00±0.00Aa	0.00±0.00Aa	0.00±0.00Aa
0.20	11.69±0.34Ba	7.74±0.17Bc	3.89±0.07Bd	4.17±0.06Bd	3.52±0.09Bd	9.38±0.17Bb
0.25	22.76±0.32Ca	7.94±0.32Bd	12.92±0.64Cb	4.98±0.34Cf	6.02±0.13Ce	9.38±0.16Bc
0.30	29.22±0.91Da	19.19±0.50Cc	16.96±0.60Dd	7.89±0.34De	7.86±0.35De	21.00±0.51Cb
0.35	34.86±0.74Ea	26.12±0.83Db	19.05±0.28Ed	9.5±0.28Ee	11.36±0.27Ee	24.00±0.78Dc

性状 Traits	EMS处理浓度Treatments concentration/%
性状 Traits	1121R	LgB7B大粒高 LgB7B Daligao	L早615·L17 L Zao 615·L17	低秆L17 Digan L17	L17	28R·吉16 28R·Ji16
成苗率Seeding survival rate	0.30	0.25	0.35	>0.35	0.35	0.25
育性 Fertility	0.25	0.30	0.25	>0.35	0.35	0.25
综合Comprehensiveness	0.25	0.25	0.25	>0.35	0.35	0.25

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[2]	LI Peng-cheng, ZHANG Ming-jun, WANG Yin-xiao, LI Xiang-yin, LI Sheng-yan, LANG Zhi-hong. Insect Resistance Identification and Agronomy Traits Analysis of Transgenic Maize HGK60 with Different Genetic Backgrounds [J]. Biotechnology Bulletin, 2023, 39(1): 40-47.
[3]	WANG Cui-cui, FU Da-qi. Research Progress in the Effects of Ubiquitin-proteasome System on Plant Agronomic Traits [J]. Biotechnology Bulletin, 2023, 39(1): 72-83.
[4]	SUN De-quan, LU Xin-hua, LI Wei-ming, HU Yu-lin, DUAN Ya-jie, PANG Zhen-cai, HU Hui-gang. Application of Mesoporous Silica Nanoparticles in Agriculture [J]. Biotechnology Bulletin, 2022, 38(5): 228-239.
[5]	ZHANG Hai-miao, LI Yang, LIU Hai-feng, KONG Ling-guang, DING Xin-hua. Research Progress on Regulatory Genes of Important Agronomic Traits and Breeding Utilization in Rice [J]. Biotechnology Bulletin, 2020, 36(12): 155-169.
[6]	LI Ai-guo, LI Ji-ming, LI He-ping, LIU Gui-hua, SONG Cong-min, WU Jun-yan. Selection of Winter Rape Varieties Suitable for Cold and Dry Areas in Hebei Province [J]. Biotechnology Bulletin, 2020, 36(1): 95-100.
[7]	DUAN Min-jie, YI Hong-wei, WANG Jin, WU Zheng. Cloning and Expression Analysis of the Pear Black Spot Resistant Gene PpEMS1 [J]. Biotechnology Bulletin, 2019, 35(11): 16-21.
[8]	HU Ya-ping, ZHOU Xu, CHEN Shui-fei, GE Xiao-min, DING Hui. Effects of Plant Virus Infection on Biological Factors and Their Interactions in the Ecosystem [J]. Biotechnology Bulletin, 2019, 35(10): 180-188.
[9]	LIANG Hai-sheng, LI Meng-tao, LI Sheng-yan, WANG Hai, ZHANG Jie, LANG Zhi-hong. Agronomic Traits Analysis of Transgenic Bt cry1Ah Maize HGK60 Line [J]. Biotechnology Bulletin, 2018, 34(7): 92-100.
[10]	LI Su-zhen, YANG Wen-zhu, CHEN Ru-mei. An Overview on Yellow Green Leaf Mutants in Rice [J]. Biotechnology Bulletin, 2018, 34(11): 15-21.
[11]	SONG Yan-chao, An Fei-fei, Xue Jing-jing, Qin Yu-ling, Li Kai-mian, CHEN Song-bi. Proteomic Analysis on Tuberous Roots of Cassava Cultivar ZM-Seaside and Mosaic-leaf Mutation [J]. Biotechnology Bulletin, 2017, 33(3): 78-85.
[12]	WANG Wei-guo, DENG Qian, JI Qiao-ling, WANG Wei. Orthogonal Test of EMS Mutation and NaCl Stress and Screening of Salt-tolerant Fiber Flax Germplasm in Vitro [J]. Biotechnology Bulletin, 2016, 32(6): 103-110.
[13]	Wang Chunyu,Zhu Zhenxing, Li Dan, Cong ling,Zhang Lixia. EMS Mutagenesis，Mutant Screening and Identification of Sorghum [J]. Biotechnology Bulletin, 2014, 0(9): 78-83.
[14]	Liu Hongquan, Lin Xiaoyuan, Li Jieqiong, Pan Yihua. The Research on Mutagenesis Breeding of Phaeodactylum tricornutum and the Condition of EPA Producing [J]. Biotechnology Bulletin, 2014, 0(9): 114-119.
[15]	Dai Jun, Li Xiuying, Zhu Li, Wang Hai, Zhang Jie, He Kanglai, Lang Zhihong, Huang Dafang. Molecular Detection and Agronomic Traits Analysis of Insect-resistant Transgenic Maize Harboring Bt cry1Ah Gene [J]. Biotechnology Bulletin, 2014, 0(5): 62-68.