Cloning and Functional Prediction of the Global Regulator FocVeA Gene from Fusarium oxysporum f. sp. cubense

Abstract

Abstract: Based on the conserved amino acid sequence of veA homologs from F. verticillioides and F. fujikuroi, FocVeA gene and its open reading frame of F. oxysporum f. sp. cubense race 1 and race 4（Foc1 and Foc4）were cloned by PCR and RT-PCR. Sequences characterization, phylogenetic clustering and conserved domains on the two predicted proteins of FocVeA gene were also analyzed, respectively. The results showed that the complete DNA sequences of FocVeA from Foc1 and Foc4（named as F1VeA and F4VeA）were 1 693 bp and 1 690 bp, respectively. The cDNA of F1VeA and F4VeA were 1 599 bp and 1 596 bp, and encoded a deduced protein of 532 amino acid and 531 amino acid, respectively. The deduced amino acid sequences of F1VeA and F4VeA shared 99% similar to each other, and had 78%-99% similarity with the sequences of veA factor from isolates of Fusarium spp., respectively. Phylogenetic clustering suggested that the amino acid sequences of F1VeA and F4VeA showed high similarity to veA homologs of F. verticillioides and F. fujikuroi deposited in GenBank. Conservative structure domain analysis showed that F1VeA and F4VeA had the sequence characteristics of veA gene family in filamentous fungi, which meant that FocVeA might involve in morphological development, toxin biosynthesis and pathogenicity of F. oxysporum f. sp. cubense.

Key words: Fusarium oxysporum f. sp. Cubense, FoVeA gene, Cloning, Sequence analysis

Qi Yanxiang, Zhang Xin, Lu Ying, Zhang He, Pu Jinji, Yu Qunfan, g Xie Yixian. Cloning and Functional Prediction of the Global Regulator FocVeA Gene from Fusarium oxysporum f. sp. cubense[J]. Biotechnology Bulletin, 2014, 0(10): 101-106.

References

[1] O, Krappmann S, Ni M, et al. VelB/veA/LaeA complex coordinates light signal with fungal development and secondary metabolism[J]. Science, 2008, 320：1504-1506.
[2] HS, Han KY, Kim KJ, et al. The veA gene activates sexual development in Aspergillus nidulans[J]. Fungal Genetics and Biology, 2002, 37：72-80.
[3] RM, Cary JW, Calvo AM. Production of cyclopiazonic acid, aflatrem, and aflatoxin by Aspergillus flavus is frgulated by veA, a gene necessary for sclerotial formation[J]. Applied Microbiology and Biotechnology, 2007, 73（5）：1158-1168.
[4] A M, Bok J, Brooks W, et al. veA is required for toxin and sclerotial production in Aspergillus parasiticus[J]. Applied and Environmental Microbiology, 2004, 70（8）：4722-4739.
[5] K, Li S, Butchko RA, et al. FvVE1 regulates biosynthesis of the mycotoxins fumonisins and fusarins in Fusarium verticillioides[J]. Journal of Agricultural and Food Chemistry, 2009, 57：5089-5094.
[6] K, Zitomer NC, Duvall M, et al. The conserved global regulator VeA is necessary for symptom production and mycotoxin synthesis in maize seedlings by Fusarium verticillioides[J]. Plant Pathology, 2012, 61（1）：152-160.
[7] J, Liu X, Yin Y, et al. Involvement of a velvet protein FgVeA in the regulation of asexual development, lipid and secondary metabolisms and virulence in Fusarium graminearum[J]. PLoS One, 2011, 6（11）：e28291.
[8] J, Urban M, Dufresne M, et al. The velvet gene, FgVe1, affects fungal development and positively regulates trichothecene biosynthesis and pathogenecity in Fusarium graminearum[J]. Molecular Plant Pathology, 2011, 4：363-374.
[9] P, Brown DW, Kleigrewe K, et al. FfVel1 and FfLae1, components of a velvet-like complex in Fusarium fujikuroi, affect differentiation, secondary metabolism and virulence[J]. Molecular Microbiology, 2010, 77（4）：972-994.
[10] YE, Goodwin SB. MVE1, encoding the velvet gene product homolog in Mycosphaerella graminicola, is associated with aerial mycelium formation, melanin biosynthesis, hyphal swelling, and light signaling[J]. Applied and Environmental Microbiology, 2011, 77：942-953.
[11] J, Eichhorn H, Friedlin E, et al. A homologue of the Aspergillus velvet gene regulates both cephalosporin C biosynthesis and hyphal fragmentation in Acremonium chrysogenum[J]. Applied and Environmental Microbiology, 2007, 73（10）：3412-3422.
[12] RB. The production and significance in phytopathology of toxins produced by species of Fusarium[C]. British Mycological Society Symposium Series, 1984：256-262.
[13] 兀旭辉, 林成辉.香蕉枯萎病菌粗毒素的毒性及其模型[J].热带作物学报, 2004, 25（4）：25-29.
[14] 黎永坚, 于莉, 等.香蕉枯萎病菌毒素的成分分析及其生物测定[J].果树学报, 2010, 6：115-120.
[15] 陈石, 左存武, 等.香蕉枯萎病菌新毒素—白僵菌素的鉴定[J].园艺学报, 2011, 38（11）：2092-2098.
[16] 张欣, 蒲金基, 等.10种化合物对香蕉枯萎病菌的抑菌作用及对毒素钝化的效果[J].果树学报, 2008, 25（1）：78-82.
[17] 曾蕊, 李瑜婷, 等.香蕉枯萎病菌4号生理小种毒素解毒剂的筛选及对香蕉防御酶活性的影响[J].果树学报, 2012, 29（1）：99-104.
[18] 舒灿伟, 陈健仪, 等.利用甲基磺酸乙酯和枯萎病菌毒素诱变筛选香蕉抗毒素突变体[J].园艺学报, 2012, 39（8）：1465-1470.
[19] 庄楚雄, 姜子德.香蕉枯萎病菌4号生理小种致病相关基因foABC1的分离[J].菌物研究, 2006, 4（3）：94-95.
[20] O, Braus GH. Coordination of secondary metabolism and development in fungi：the velvet family of proteins[J]. FEMS Microbiology Reviews, 2012, 35：1-24.
[21] N, Brooks W, Clavo AM. The expression of sterigmatocystin and penicillin genes in Aspergillus nidulans is controlled by veA, a gene required for sexual development[J]. Eukaryotic Cell, 2003, 2（6）：1178-1186.
[22] Aaike S, Keller NP. Distinct roles for VeA and LaeA inn development and pathogenesis of Aspergillus flavus[J]. Eukaryot Cell, 8：1051-1060.