Cloning,Sequence and Tissue Expression Pattern Analysis of HOXC10 Gene in Multi-costa Properties Yaks of Jinchuan

Abstract

Abstract: The present study aimed at cloning yak HOXC10 gene, and further analysis of the gene structure and function, and reveals the tissue specificity expression pattern. The Jinchuan multi-costa properties yak as materials, this study based on the GenBank published Ovis aries HOXC10 gene sequences for primier designing, using RT-PCR technology to amplification the cDNA full length of HOXC10 gene, and its bioinformatic analysis and expression profiling in various tissues. The sequencing result showed that the length of yak HOXC10 gene is 1 272 bp and contains a 1 029 bp open reading frame（ORF）, encoding 342 amino acids. The results of homology analysis showed that yak and bos taurus has the higher similarity（93.2%）;Followed by predicting HOXC10 protein molecular weight 38.2 kD, the theory of isoelectric point is
8.45. Evolutionary tree analysis results showed that yak was closest relative with bos taurus, and in the same branch. Tissue expression profile analysis showed that HOXC10 gene is expressed in different degrees in various organizations, which had the highest expression in the liver, the lowest expression in the stomach.

Key words: Yak, Cloning, HOXC10, Sequence analysis, Tissue expression

Zhang Yanan, Xiong Xianrong, Lan Daoliang, Fu Mei, Zhang Yan, Hou Dingchao, Ma Dingmei, Li Shanrong, Li Jian. Cloning,Sequence and Tissue Expression Pattern Analysis of HOXC10 Gene in Multi-costa Properties Yaks of Jinchuan[J]. Biotechnology Bulletin, 2014, 0(10): 139-143.

References

[1] HOX genes in vertebrate development[J]. Cell, 1994, 78（2）：191-201.
[2] U, Sauvageau G, Hough MR, et al. Overexpression of HOXA10 in murine hematopoietic cells perturbs both myeloid and lymphoid differentiation and leads to acute myeloid leukemia[J]. Mol Cell Biol, 1997, 17（1）：495-505.
[3] MR, Komine Y, Bryant SV, et al. Expression of Hoxb13 and Hoxc10 in developing and regenerating axolotl limbs and tails[J]. Dev Biolo, 2001, 229：396-406.
[4] Christen B, Beck CW, Lombardo A, et al. Regeneration-specific expression pattern of three posterior Hox genes[J]. Dev Dyn, 2003, 226：349-355.
[5] 张倡珲, 郑玉才, 等. 九龙牦牛肌细胞生成素基因的克隆及其表达谱[J].畜牧兽医学报, 2014, 45（2）：207-211.
[6] G, Han JL, Long RJ. The Yak[M]. 2nd edition. FAO, 2003.
[7] 熊显荣, 李宇, 等. Scriptaid处理牦牛成纤维细胞对组蛋白乙酰化及重编程的影响[J].农业生物技术学报, 2013, 21（1）：62-68.
[8] 张立岭, 陈琦, 等.蒙古羊HOXC8基因甲基化与胸椎数量的关系[J].黑龙江畜牧兽医, 2011, 5：11-14.
[9] 方美霞, 何小梅, 等.猪繁殖候选基因HOXA10的克隆及表达分析[J].湖南农业大学学报：自然科学版, 2011, 37（1）：60-64.
[10] ES, Yan LJ, Benjamin IJ. Heat shock factor 1 and heat shock proteins：critical partners in protection against acute cell injury[J]. Critical Care Med, 2002, 30（1）：S43-S50.
[11] Lewis EB. Clusters of master control genes regulate the development of higher organisms[J]. JAM, 1992, 267（11）：1524-1531.
[12] Ferrier DEK. HOX genes：Did the vertebrate ancestor have a HOX14?[J]. Current Biology, 2004, 14：210-211.
[13] S, Naora H. Homeobox gene expression in cancer：insights from developmental regulation and deregulation[J]. Eur J Cancer, 2005, 41：24-28.
[14] 1992, 15（11）：10-13.
[15] 熊显荣, 兰道亮, 等.麦洼牦牛HOXA10基因的克隆、原核表达及组织表达谱[J].西北农业学报, 2013, 22（7）：7-11.
[16] EA, Stocum DL, Nye HL, et al. Urodele spinal cord regeneration and related processes[J]. Dev Dyn, 2003, 226：295-307.
[17] HOXC8在小鼠成骨细胞分化中的调控用作研究[D].长春：吉林大学, 2009.
[18] A, Kaczmarek MM, Kiewisz J, et al. Endometrialand conceptus expression of HOXA10, transforming growth factor beta1, leukemia inhibitory factor, andprostaglandin H synthase-2 in early pregnant pigs with gonadotropin-induced estrus[J]. Domest Anim Endocrinol, 2010, 38（4）：222-234.
[19] 熊显荣, 高川, 等.麦洼牦牛乳腺上皮细胞系的建立及其生物学特性[J].西北农业校报, 2012, 21（11）：8-13.