Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (3): 185-190.doi: 10.13560/j.cnki.biotech.bull.1985.2015.04.027

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Optimization of Extract Condition of Safflower Oil Body and Analysis of Stability

Yang Jing1,2 Han Gaoqiang2 Liu Zhongliang2 Lu Zhen2 Wu Zhibang2 Wang Qingman2 Zou Deyi2 Qiang Weidong2 Chen Wei2   

  1. (1. Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development,Changchun 130118;2. College of Life Sciences,Jilin Agricultural University,Changchun 130118)
  • Received:2014-08-04 Online:2015-03-16 Published:2015-03-16

Abstract: In order to achieve a wide range of applications in many fields, especially on oil body as a substrate carrier system of the foundation, extraction method of safflower oil and characteristics of safflower oil body lotion was researched. Extraction efficiency of safflower oil body was compared, including its value at different pH, NaCl concentration conditions, an average particle size of safflower oil and stability, as well as its viscosity to conduct investigations. Results showed that the safflower oil body dispersed more evenly, at pH≥6, the average particle size of 1.75-2.05 μm and pH ≤ 6 conditions, the average particle size of 1.50-1.75 μm; while NaCl concentration of 0.2 and 0.4 mg/mL, NaCl concentration 1.2 and 2.0 mg/mL, the safflower oil bodies appeared aggregation. When sucrose concentration between 0.1 and 0.2 mg/mL, safflower oil dispersion is uniform; when the sugar concentration of 0.4-1.0 mg/mL, safflower body intensive, with increasing concentration of sucrose, safflower oil body size gradually began to heterogeneity. The optimum extraction condition for safflower oil body is pH7, NaCl 0.2 mg/mL, sucrose concentration of 0.1 mg/mL. The study proved that safflower oil body without adding detergent or without physical approach, the saved is unstable.

Key words: safflower, oil-body, extraction, size, stability