Study on Antibacterial Stability of Musca domestica Cecropin-MDC Against Salmonella typhimurium

doi:10.13560/j.cnki.biotech.bull.1985.2021-0847

Abstract

Abstract:

This work aims to investigate the antibacterial stability of the housefly antimicrobial peptide（Musca domestica cecropin,MDC）against Salmonella typhimurium under different conditions. The Oxford cup method was used to determine the influences of pH,temperature,light,protease,metal ions,surfactants and organic reagents on the antibacterial activity（bacteriostatic ring size）of MDC against S. typhimurium. The results showed that the activity of MDC decreased slightly under acidic condition（remained 85% of that under the normal condition）,but not changed under alkaline condition with a good stability. Under low or high temperature treatment,no obvious difference was observed in its antibacterial activity. In addition,the activity of MDC still remained stable after exposed to darkness,normal light or ultraviolet for 30 min. MDC was sensitive to trypsin and its activity completely disappeared,but good stability to pepsin. MDC was still stable in the Ca²⁺ and K⁺,but it was sensitive to Mg²⁺. Interestingly,the antibacterial activity of MDC under Fe³⁺increased compared with the control group（P<0.05）. Moreover,the antimicrobial activities of MDC treated with surfactant and organic reagents significantly reduced but still partially preserved. To sum up,MDC can significantly inhibit the growth and reproduction of S. typhimurium,and its activity is almost not affected by the change of pH value/temperature/light and different pepsin and metal ions. Ferric ion can synergistically enhance the antibacterial activity of MDC,but Mg²⁺,surfactant,organic reagent and trypsin hydrolysis caused its antibacterial activity partially or completely lost,respectively.

Key words: Musca domestica cecropin, stability, Salmonella typhimurium, antibacterial activity

WANG Zi-yan, WANG Jian, ZHANG Lun, GUI Shui-qing, LU Xue-mei. Study on Antibacterial Stability of Musca domestica Cecropin-MDC Against Salmonella typhimurium[J]. Biotechnology Bulletin, 2022, 38(3): 149-156.

Figures/Tables 9

Fig.1 HLPC of MDC

Fig.2 MS analysis of MDC

Fig. 3 Antibacterial activity of MDC on S. typhimurium after different pH treatment All the samples in the bacteriostatic plate are numbered clockwise. 1,2,3 and 4 represent normal saline,treated MDC,ceftriaxone sodium and untreated MDC respectively. Compared with the control group（untreated MDC）,*P < 0.05,**P < 0.01,n=3

Fig. 4 Antibacterial activity of MDC on S. typhimurium after different temperature treatment All the samples in the bacteriostatic plate are numbered clockwise. 1,2 and 3 are normal saline,MDC treated with different temperature and ceftriaxone sodium respectively,n=3

Fig. 5 Antibacterial activity of MDC on S. typhimurium under different light condition All the samples in the bacteriostatic plate are numbered clockwise. 1,2 and 3 are normal saline,MDC treated with different light conditions and ceftriaxone sodium respectively,n = 3

Fig. 6 Antibacterial activity of MDC on S. typhimurium after different proteases treatment All the samples in the bacteriostatic plate are numbered clockwise. 1,2,3 and 4 represent normal saline,MDC treated with protease,ceftriaxone sodium and MDC treated at 95℃ respectively. Compared with the control group（MDC treated at 95℃）,****P < 0.0001,n = 3

Fig. 7 Antibacterial activity of MDC on S. typhimurium after different metal ion treatment All the samples in the bacteriostatic plate are numbered clockwise. 1,2,3 and 4 represent normal saline,untreated MDC,ceftriaxone sodium and MDC treated with metal ion respectively. Compared with the control group（untreated MDC）,***P < 0.001,n = 3

Fig. 8 Antibacterial activity of MDC on S. typhimurium after different surfactant treatment All the samples in the bacteriostatic plate are numbered clockwise. 1,2,3 and 4 represent normal saline,untreated MDC,ceftriaxone sodium and MDC treated with surfactant respectively. Compared with the control group（untreated MDC）,***P < 0.001,n = 3

Fig. 9 Antibacterial activity of MDC on S. typhimurium after different organic solvent treatment All the samples in the bacteriostatic plate are numbered clockwise. 1,2,3 and 4 represent normal saline,untreated MDC,ceftriaxone sodium and MDC treated with organic solvent respectively. Compared with the control group（untreated MDC）,***P < 0.001,n = 3

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