Isolation，Culture and Identification of Beijing Duck Embryonic Metanephric Mesenchymal Stem Cells

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.028

Abstract

Abstract: This study aimed to establish in vitro culture system for the poultry metanephric mesenchymal stem cells(MMSCs), and study their biological characteristics and multi-directional differentiation potential. Enzyme digestion method was applied to separate Beijing duck embryo MMSCs and to draw the growth curve. MMSCs were identified by immunofluorescence and RT-PCR. MMSCs were induced to differentiate into adipocytes and islet cells. The results showed that MMSCs had solid proliferative activity and could express MSCs specific markers, and be induced to differentiate into adipocytes and islet cells. In summary, Beijing duck embryo MMSCs have a strong self-renewing ability and multi-directional differentiation potential in vitro, and can be saved as seed cells for tissue engineering.

Key words: duck, metanephric mesenchymal stem cell, biological characteristics, induced differentiation

Chen Jia, Wang Guiyan, Zhang Yu. Isolation，Culture and Identification of Beijing Duck Embryonic Metanephric Mesenchymal Stem Cells[J]. Biotechnology Bulletin, 2015, 31(6): 177-182.

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