Constructing a Human Oocyte Reporter System

doi:10.13560/j.cnki.biotech.bull.1985.2015.11.033

Abstract

Abstract: In vitro differentiation of stem cells into mature germ cells has important significance both in scientific research and in clinical treatment. An oocyte reporter system will become a useful tool for this goal. ZP2 is a component of the zona pellucida in mammalian oocytes and its expression is known to be restricted in oocyte. In this experiment we cloned 300 bp and 2 500 bp fragment of human ZP2 promoter and fused them to eGFP aiming to build an oocyte reporter sysem. We tested the specificity of the reporter in WI38, undifferentiated human embryonic stem cell(hESC)line H9 and mouse oocyte. Results show that ZP2-2500-eGFP can specifically drive eGFP expression in mouse oocyte but no eGFP was detected in WI38 and undifferentiated hESCs.

Key words: reporter system, ZP2, oocyte

Luo Mengyuan, Kee Kehkooi. Constructing a Human Oocyte Reporter System[J]. Biotechnology Bulletin, 2015, 31(11): 251-256.

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