Abstract: With the depletion of fossil fuels and the worsening of environmental pollution,clean and renewable energies such as biofuels have become a hot topic for research all over the world. Biobutanol becomes a promising one among the new generation of biofuels because of its high energy density,high combustion value and light pollution. Nevertheless,the high production cost of traditional Acetone-Butanol-Ethanol（ABE）fermentation and the low production rate limited the commercial production of biobutanol. In order to reduce the cost of feedstock and to achieve the effective industrial transformation of cheap biomass,many researches focus on economic fermentation technology based on biomass resources. By exogenous adding technology,strain phenotype and fermentation performance were revealed rapidly in fermentation system,which contributed to the explanation of interactions in the strain metabolic level and gene level. In addition,with the development of genome sequencing and omics engineering technology,an increasing number of researches aimed to improve the metabolic synthesis ability and the tolerance of biobutanol were reported. These researches included transforming metabolic network structure,blocking non-butanol metabolism pathway,clarifying the mechanism of regulations to stress,and removing related metabolic regulation,which were for endogenous transformation of butanol-producing Clostridium. In this paper,based on the development of biobutanol fermentation biomass resources,macro-control strategy of metabolism and strain breeding,the bottleneck in the process of biobutanol production and metabolism was discussed,and the biobutanol development was also prospected. The aim is to provide theoretical basis for the construction of engineering bacteria and metabolic regulation based on process engineering technology.

Key words: biobutanol, ABE fermentation, biomass resources, metabolic regulation, genetic engineering