Detection of Quorum Sensing Signal Molecules in Vibrio anguillarum Isolated From Litopenaeus vannamei

doi:10.13560/j.cnki.biotech.bull.1985.2016.03.023

Abstract

Abstract: The aim of the present study was to detect and analyze quorum sensing of Vibrio anguillarum(VA)isolated from spoiled Litopenaeus vannamei. AHLs activities of VA were detected by reporter strains(Chromobacterium violaceum CV026 and Agrobacterium tumefaciens A136)combined with TLC method. Report strains V. harveyi JMH597 and V. harveyi JAF375 were used to detect the activity of AI-2 and CAI-1. The result indicated that AHLs(3-OH-C₆-HSL, C₈-HSL and 3-oxo-C₈-HSL)、AI-2 and CAI-1 were secreted by VA andthe activity of the signal molecules were density-dependent.

Key words: Vibrio anguillarum, Quorum sensing, AHLs, AI-2, CAI-1

PAN Yu-rong, ZHANG Cai-li, ZHU Su-qin, SUN Xiu-jiao, ZENG Ming-yong. Detection of Quorum Sensing Signal Molecules in Vibrio anguillarum Isolated From Litopenaeus vannamei[J]. Biotechnology Bulletin, 2016, 32(3): 142-147.

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URL: https://biotech.aiijournal.com/EN/10.13560/j.cnki.biotech.bull.1985.2016.03.023

https://biotech.aiijournal.com/EN/Y2016/V32/I3/142

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Detection of Quorum Sensing Signal Molecules in Vibrio anguillarum Isolated From Litopenaeus vannamei

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[1]	HUANG Ya-ning, ZHANG Hai-jiao, HAN Yu-qian, LIU Zun-ying. Effects of Supercritical CO₂ Combined with Ginger Essential Oil on the Sterilization of Vibrio parahaemolyticus and Its Mechanism [J]. Biotechnology Bulletin, 2023, 39(5): 297-305.
[2]	HUANG Xiang-mei, WU Ya-qian, LIU Ying, LIANG Jia-ye, SU Wei-ming. Inhibitory Effects of AI-2 Quorum Sensing Inhibitors from Marine Lactic Acid Bacteria on Listeria monocytogenes [J]. Biotechnology Bulletin, 2019, 35(4): 36-42.
[3]	ZHOU Heng, JIANG Yun, XU Ye-xiang, QIAN Sheng-hui, MIAO Li. Research on Quorum Sensing Inhibitory Activity and Culture Condition of a Marine Streptomyces parvulus [J]. Biotechnology Bulletin, 2019, 35(10): 137-143.
[4]	CHEN Shao-wei, WU Cheng, SU Yue-hua, CAI Bin-bin, XIE Pan-pan, YANG Mei. Cloning and Functional Identification of the 5' flanking Region of the aiiA Gene from Bacillus thuringiensis [J]. Biotechnology Bulletin, 2018, 34(11): 136-143.
[5]	MAO Wen, TIAN Lu, GONG Guo-li. Research Progress on Bacteriocins and Their Potential New Applications [J]. Biotechnology Bulletin, 2018, 34(10): 35-40.
[6]	PAN Yu-rong, ZHANG Cai-li, ZHU Su-qin, ZENG Ming-yong. Inhibition of Brominated Furanone to Quorum Sensing Regulating Behaviors of Vibrio anguillarum [J]. Biotechnology Bulletin, 2017, 33(4): 231-237.
[7]	FAN Xing-hui, WANG Hui-shan HE, Jie-hua YE, Tian YANG, Fang CHEN Shao-hua. Research Progress on Microbial Quorum Quenching Enzymes and Their Control of Plant Diseases [J]. Biotechnology Bulletin, 2017, 33(10): 80-87.
[8]	ZHANG Shu-mei,XU Xiang-rong,XU Hao. Research Progress on Quorum Sensing System of Bacterial Biofilm [J]. Biotechnology Bulletin, 2016, 32(12): 19-22.
[9]	JIN Xiao-yang, HOU Peng-fei, ZHANG Xiao-han, ZHAO Qian, SONG Shui-shan. Advances on Regulation of Plant Disease Resistance by N-acyl-homoserine Lactones of a Bacterial Signal Molecule [J]. Biotechnology Bulletin, 2016, 32(11): 47-51.
[10]	Ai Qiushi, Zhang Zhe, Qu Lingbo, Liu Fang, Zhao Qian, Song Shuishan. Preliminarily Research on Involvement of Arabidopsis GCR2 Responsing to N-Butyryl-DL-homoserine Lactone [J]. Biotechnology Bulletin, 2015, 31(8): 94-101.
[11]	Xing Qifan, Liu Pengfu, Shi Jiping, Sun Yumei. Research Progress on Bacterial Quorum Quenching Enzymes [J]. Biotechnology Bulletin, 2015, 31(10): 48-55.
[12]	Liang Xinyan, Ruan Haihua. Quorum Sensing and Its Application in Preventing and Therapeutic Effect for Pathogenic Bacteria [J]. Biotechnology Bulletin, 2015, 31(1): 33-38.
[13]	Zhang Caili, Zhu Suqin, Wang Ying, Sun Xiujiao, Zeng Mingyong. Isolation, Identification and Quorum Sensing of a Serratia spp. [J]. Biotechnology Bulletin, 2014, 0(7): 150-155.
[14]	Huang Yuanyuan, Ma Hong, Jia Zhenhua, Huang Yali , Song Shuishan, Zhang Xia. Isolation and Identification of Accumulation of Quorum Signal Molecules Strains from the Trichoplusia ni Midgut [J]. Biotechnology Bulletin, 2014, 0(7): 196-200.
[15]	Wang Ying, Zhu Suqin, Zhang Caili, Zeng Mingyong. Detection of Quorum Sensing in Vibrio parahaemolyticus Isolated from Macrobrachium rosenbergii [J]. Biotechnology Bulletin, 2014, 0(3): 146-150.