RAPD Analysis of Genetic Diversity in Saccharina japonica Germplasm Resources

doi:10.13560/j.cnki.biotech.bull.1985.2016.04.020

Abstract

Abstract: The genetic diversity of 36 gametophytes of 19 varieties（or lines）of Saccharina japonica was analyzed with RAPD（random amplified polymorphic DNA）markers. Sixteen primers with clear bands were selected out from 100 primers for diversity analysis. These primers amplified 362 DNA bands from 36 gametophytes，and 99.45% of the bands were polymorphic. The genetic similarity of the 36 gametophytes ranged from 0.682-0.978. The 36 gametophytes germplasm were clustered into 6 groups while 0.756 was as the minimum genetic similarity coefficient. The UPGMA cluster results indicated that the male and female gametophyte of the same species were clustered together，and genetic diversity among species was high.

Key words: kelp, gametophyte, RAPD, genetic diversity

LI Yan, LIU Yan-ling, CUI Cui-ju ,LI Xiao-jie, LIANG Guang-jin ,PENG Jie ,TIAN Ping-ping. RAPD Analysis of Genetic Diversity in Saccharina japonica Germplasm Resources[J]. Biotechnology Bulletin, 2016, 32(4): 151-158.

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